J Korean Ophthalmol Soc.
1992 Dec;33(12):1194-1199.
Distribution of Phospholipase C Isozymes in Rat Retina: Immunohistochemical Study
- Affiliations
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- 1Department of Ophthalmology, College of Medicine, Gyeong Sang National University, Korea.
- 2Department of Anatiomy, College of Medicine, Gyeong Sang National University, Korea.
- 3Kyungsang Plant Molecular Biology and BioTechnology Reserch Center, Korea.
Abstract
- In order to explore the existence and distribution of phospholipase (PLC) isozymes in the rat retina, immunohistochemical staining was applied using monoclonal antibodies against PLC isozymes (PLC beta; K92, PLC gamma; D7, F7, PLC delta; R32, S11). For immunohistochemical detection, avidin-biotin peroxidase complex (ABC) method was performed on frozed tissue sections of rat retina. Our study showed that PLC isozymes have particular distributional patterns in the retina. Namely, PLC beta is broadly distributed in the outer and inner segments of photoreceptor cell layer, nuclear layer and ganglion cell layer. PLC gamma is mainly appeared in the nerve fiber layer, ganglion cell layer and inner nuclear layer. PLC delta is confined only in the ganglion cell layer. These results clearly demonstrate the PLC isozymes may have their own role in the transduction of light pathway in the retina. However, further studies will be required to verify theirs precise role in the photoreception.
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