Korean J Anesthesiol.  2006 Jun;50(6):706-713. 10.4097/kjae.2006.50.6.706.

Effect of the Heme Oxygenase Inhibitor on the Hypoxic Ischemic Brain Injury in the Neonatal Rat

• Bang JY
• Park PH

• KMID: 1862249
• DOI: http://doi.org/10.4097/kjae.2006.50.6.706

Abstract

BACKGROUND: The heme oxygenase system catalyzes the conversion of heme to free iron, carbon monoxide and bilirubin. This study was purposed to evaluate the effect of the heme oxygenase inhibitor, Tin protoporphyrin IX (SnPPIX) on the hypoxic ischemic brain injury in a neonatal rat.
METHODS
Seven-day old Sprague-Dawley rat pups were used. The rats were divided into two groups; control group (n = 9) and SnPPIX group (n = 6). SnPPIX 50 micromol/kg and the dissolvent were administered respectively intraperitoneally. For hypoxic ischemic brain injury, the right common carotid artery was ligated with 5-0 silk and the rats were put in the moisturized hypoxic gas chamber for 150 minutes. Lipid/N-acetyl aspartate and Lipid/Creatine ratio of 1H magnetic resonance spectroscopy were evaluated on the 1st day and the 7th day after hypoxic ischemic brain injury. All rats were sacrificed 2 weeks after hypoxic ischemic brain injury for morphological study.
RESULTS
There were no statistically significant differences between two groups in the result of MRS and Lip/Cr and Lip/NAA ratio on 1th day and 7th day after hypoxic ischemic brain injury. In addition, there was no significant difference in the gross morphological evaluation on the 14th day.
CONCLUSIONS
Our results suggest that the pretreatment of the Tin protoporphyrin IX does not affect the degree of brain damage which is caused by apoptosis in the model of hypoxic ischemic brain injury in a neonatal rat.

Keyword

heme oxygenase; heme oxygenase inhibitor; 1H-magnetic resonance spectroscopy; hypoxic ischemic brain injury

MeSH Terms

Animals
Apoptosis
Aspartic Acid
Bilirubin
Brain Injuries*
Brain*
Carbon Monoxide
Carotid Artery, Common
Heme Oxygenase (Decyclizing)*
Heme*
Iron
Magnetic Resonance Spectroscopy
Rats*
Rats, Sprague-Dawley
Silk
Tin
Aspartic Acid
Bilirubin
Carbon Monoxide
Heme
Heme Oxygenase (Decyclizing)
Iron
Silk
Tin