J Korean Soc Traumatol.  2008 Dec;21(2):120-127.

Time Course of Inducible NOS Expression of Lung Tissue during Sepsis in a Rat Model

• Kim JH
• Kim SC
• Kwon WY
• Suh GJ
• Youn YK

Abstract

PURPOSE: Many studies on the time course of inducible nitric oxide synthase (iNOS) gene expression have been performed in the LPS (Lipopolysaccharide)-induced endotoxemic model, but there have been few experimental approaches to continuous peritonitis-induced sepsis model. We conducted this study to establish basic data for future sepsis-related research by investigating the time course of iNOS gene expression and the relationship with the production of inflammatory mediators in the early sepsis model induced by cecal ligation and puncture (CLP).
METHODS
Male Sprague-Dawley rats were operated on by sing the CLP method to induce of peritonitis; and then, they were sacrificed and samples of blood and lung tissues were obtained at various times (1,2,3,6,9 and 12 h after CLP). We observed the expression of iNOS mRNA from lung tissues and measured the synthesis of nitric oxide, IL-1beta , and TNF-alpha from the blood.
RESULTS
iNOS mRNA began to be expressed at 3 h and was maintained untill 12 h after CLP. The nitric oxide concentration was increased significantly at 6 h, reached its peak level at 9 h, and maintained a plateau untill 12 h after CLP. TNF-alpha began to be detected at 3 h, increased gradually, and decreased steeply from 9 h after CLP. IL-1beta showed its peak level at 6 h after CLP, and tended to decrease without significance.
CONCLUSION
We observed that the iNOS gene was expressed later in peritonitis-induced sepsis than in LPSinduced sepsis. Nitric oxide and key inflammatory mediators were also expressed later in peritonitis-induced sepsis than in LPS-induced sepsis.

Keyword

Nitric oxide synthase; Inflammation mediators; Sepsis; Peritonitis

MeSH Terms

Animals
Gene Expression
Humans
Inflammation Mediators
Ligation
Lung
Male
Nitric Oxide
Nitric Oxide Synthase
Nitric Oxide Synthase Type II
Peritonitis
Punctures
Rats
Rats, Sprague-Dawley
RNA, Messenger
Sepsis
Tumor Necrosis Factor-alpha
Inflammation Mediators
Nitric Oxide
Nitric Oxide Synthase
Nitric Oxide Synthase Type II
RNA, Messenger
Tumor Necrosis Factor-alpha