J Menopausal Med.  2015 Aug;21(2):89-92. 10.6118/jmm.2015.21.2.89.

Expression of Vitamin D Receptor in Seminal Vesicles of Cholesterol Formula Mice

• Kim TH
• Lee HH
• Kim JM
• Choi SD
• Lee A
• Hwang SY
• Kim M
• Kim YS
• Yeom S

• KMID: 2200984
• DOI: http://doi.org/10.6118/jmm.2015.21.2.89

Abstract

OBJECTIVES
Genomic function of vitamin D receptor (VDR) indicates spermatogenesis that is important for in male reproductive organ authors evaluated the VDR expression in seminal vesicles with high cholesterol (HC) formula diet rat, because there is no report about relationship or difference in VDR in seminal vesicles between HC and control.
METHODS
Male C57BL/6 mice aged 5 weeks were raised for 13 weeks. After one week of adaptation-period, they were fed different diet on normal AIN-93G diet, or HC diet containing 2% cholesterol for 12 weeks. The antibodies used were rabbit anti-VDR primary polyclonal.
RESULTS
There was no significant difference in VDR reactivity in seminal vesicles, body weight of rat and weight of seminal vesicles between HC group and normal control group.
CONCLUSION
Our data give the no difference in expression of VDR of seminal vesicles rat between HC formula diet and normal AIN-93G diet. But we confirmed the VDR expression in seminal vesicles.

Keyword

Cholesterol; Seminal vesicles; Vitamin D receptor

MeSH Terms

Animals
Antibodies
Body Weight
Cholesterol*
Diet
Humans
Male
Mice*
Rats
Receptors, Calcitriol*
Seminal Vesicles*
Spermatogenesis
Vitamin D*
Vitamins*
Antibodies
Cholesterol
Receptors, Calcitriol
Vitamin D
Vitamins

Figure

• 

  Fig. 1 Localization of vitamin D receptor in the seminal vesicle of each of groups as brown color. Immunohistochemistry was performed as described in the Material and Methods section. (A) normal control group (NC), (B, C) high cholesterol (HC) group, magnification: × 100. NC and diet on normal AIN-93G diet, HC diet on HC.

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