J Korean Soc Transplant.
2001 Dec;15(2):147-150.
The Effects of Glutathione on Cyclosporine Induced Cytotoxicity in Cultured Insulinoma Cells
- Affiliations
-
- 1Department of Surgery, College of Medicine, Chosun University, Gwangju, Korea.
Abstract
-
PURPOSE: Cycloporine A (CsA) is a immunosuppressive agent most widely used in organ transplanted patients for preventing immunorejection, but it has some side effects, including hypertension, nephrotoxicity, hepatotoxicity and diabetes. The mechanism of toxicity of CsA was not completely understood, but the reactive oxygen species has been proposed to be involved in the reaction of toxicity of CsA. The purpose of this study is to determine the effects of glutathione, as a physiological antioxidant on CsA induced cytotoxicity in rat insulinoma (RINm5F) cells.
METHODS
RINm5F cells were incubated in the presence of CsA (105~108 M) and buthionine sulfoximine (BSO), as a inhibitor of r-glutamyl cysteine synthetase, was added to cultured media (RPMI1640). Twenty four hours of incubation with CsA and BSO, viable cells were determined by MTT method.
RESULTS
CsA decreased cell viability in dose response in cultured RINm5F cells and significantly decreased according to redusing glutathione.
CONCLUSION
These results suggested that CsA may induce the diabetes and glutathione have some roles in the pathogenesis of CsA-induced diabetes.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Effects of Gutathione on Cyclosporine A-induced Cyotoxicity in Cultured Rat Insulinoma (RIN5mF) Cells
- Increased ROS Production by High Glucose in Cultured Mouse Insulinoma Cell Line
- Benzo[a]pyrene-Induced DNA-Protein Crosslinks in Cultured Human Lymphocytes and the Role of the GSTM1 and GSTT1 Genotypes
- Effects of Glutathione on Cisplatin-Induced Cytotoxicity In Human Cervical Cancer Cell Lines
- Effect of Antioxidants on FeSO4 Toxicity in Cultured Myocardial Cells
