J Korean Acad Oral Health.  2020 Dec;44(4):175-179. 10.11149/jkaoh.2020.44.4.175.

Inhibitory effect of mastic oil on Streptococcus mutans growth

Affiliations
  • 1Department of Preventive Dentistry, School of Dentistry, Kyungpook National University
  • 2Department of Dental Hygiene, College of Science & Technology, Kyungpook National University, Daegu, Korea

Abstract


Objectives
The aim of this study was to examine the antibacterial effect of mastic oil on a representative caries-inducing bacterium, Streptococcus mutans.
Methods
S. mutans UA159 was maintained in a BHI medium containing 0.1% sugar at 37℃ and 10% CO2 . Strains were treated with six different concentrations of mastic oil (0, 0.1, 0.2, 0.3, 0.4, 0.5%). After incubation for 16 h, the antibacterial effect was evaluated by measuring the optical density (OD) and observing the colony-forming unit. A microtiter plate assay was performed to analyze the anti-adhesion ability of mastic oil on S. mutans.
Results
More than 0.1% of mastic oil inhibited the growth of S. mutans. In addition, 0.4% mastic oil exterminated S. mutans. Mastic oil induced an anti-adhesive ability in S. mutans.
Conclusions
These results suggest that mastic oil may be used as a preventive measure against dental caries.

Keyword

Antibacterial effect; Mastic oil; Streptococcus mutans

Figure

  • Fig. 1 Experimental procedure.

  • Fig. 2 Identification of the number of S. mutans processed for each concentration of mastic oil in 15% sucrose MSB agar plate. (A) Negative control, (B) 0.1% mastic oil, (C) 0.2% mastic oil, (D) 0.3% mastic oil, (E) 0.4% mastic oil, (F) 0.5% mastic oil, (G) positive control.

  • Fig. 3 Adhesive ability of S. mutans with each concentration. *50% hexamedine solution. †There is significant difference between the mastic oil and the negative control groups at each concentration by Bonferroni-corrected Wilcoxon’s rank sum test (P<0.05). ‡There is significant difference between the mastic oil and the positive control groups at each concentration by Bonferroni-corrected Wilcoxon’s rank sum test (P<0.05).


Reference

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