Biomol Ther.  2013 Jan;21(1):72-78.

Anti-Inflammatory, Antioxidant, Anti-Angiogenic and Skin Whitening Activities of Phryma leptostachya var. asiatica Hara Extract

Affiliations
  • 1Department of Anatomy, Yonsei University College of Medicine, Seoul 120-749, Republic of Korea.
  • 2Korea Basic Science Institute Chuncheon Center, Chuncheon 200-701, Republic of Korea.
  • 3Shebah Biotech Inc., Chuncheon Biotechnology Foundation, Hi-Tech Venture Town, Chuncheon 200-161, Republic of Korea.
  • 4Department of Neurology, Hanyang University College of Medicine, Seoul 133-791, Republic of Korea.
  • 5Department of Biochemistry, Kangwon National University, Chuncheon 200-701, Republic of Korea. cjlim@kangwon.ac.kr

Abstract

This work aimed to assess some pharmacological activities of P. leptostachya var. asiatica Hara. The dried roots of P. leptostachya var. asiatica Hara were extracted with 70% ethanol to generate the powdered extract, named PLE. Anti-angiogenic activity was detected using chick chorioallantoic membrane (CAM) assay. In vitro anti-inflammatory activity was evaluated via analyzing nitric oxide (NO) content, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and reactive oxygen species (ROS) level in the stimulated macrophage cells. Matrix metalloproteinase-9 (MMP-9) and -2 (MMP-2) activities in the culture media were detected using zymography. PLE exhibits an anti-angiogenic activity in the CAM assay, and displays an inhibitory action on the generation of NO in the LPS-stimulated macrophage cells. In the stimulated macrophage cells, it is able to diminish the enhanced ROS level. It can potently scavenge the stable DPPH free radical. It suppresses the induction of iNOS and COX-2 and the enhanced MMP-9 activity in the stimulated macrophage cells. Both monooxygenase and oxidase activities of tyrosinase were strongly inhibited by PLE. Taken together, the dried roots of P. leptostachya var. asiatica Hara possess anti-angiogenic, anti-inflammatory, antioxidant and skin whitening activities, which might partly provide its therapeutic efficacy in traditional medicine.

Keyword

Anti-angiogenic; Anti-inflammatory; Antioxidant; Matrix metalloproteinase-9; Skin whitening; Phryma leptostachya var. asiatica Hara
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