Nat Prod Sci.  2019 Mar;25(1):34-37. 10.20307/nps.2019.25.1.34.

Isolation of Constituents with Nitric Oxide Synthase Inhibition Activity from Phryma leptostachya var. asiatica

Affiliations
  • 1College of Pharmacy, Seoul National University, Seoul 08826, Korea.
  • 2College of Oriental Medicine, Sangji University, Wonju 26339, Korea.
  • 3Department of Pharmaceutical Engineering, Sangji University, Wonju 26339, Korea. hjpark@sangji.ac.kr

Abstract

Phytochemical studies were performed to identify the active principles of Phryma leptostachya var. asiatica (Phyrymaceae) for anti-inflammation. The anti-inflammatory activity was assessed by measuring the inhibition rate on nitric oxide (NO) formation in lipopolysaccharide (LPS)-activated macrophage 264.7 cells. Of the five compounds including ursolic acid, phrymarolin I, harpagide, haedoxancoside A, and acteoside isolated from this plant, ursolic acid showed the most prominent inhibition of NO formation. Therefore, ursolic acid may be the anti-inflammatory principle of Phryma leptostachya var. asiatica.

Keyword

Phryma leptostachya var. asiatica; Phyrymaceae; nitric oxide; anti-inflammatory; ursolic acid

MeSH Terms

Macrophages
Nitric Oxide Synthase*
Nitric Oxide*
Plants
Nitric Oxide
Nitric Oxide Synthase

Figure

  • Fig. 1 Structure of compounds 1 – 5 isolated from P. leptostachya var. asiatica.

  • Fig. 2 Effect of compound 1 on NO formation in LPS-activated macrophage 264.7 cells. Cells were pretreated with different concentrations of ursolic acid and stimulated with LPS (1 µg/mL). The amount of nitrate was measured by Griess reaction. The cell viability was measured using MTT. The data are presented as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.005 by t-test.


Reference

1. Jung HJ, Cho YW, Lim HW, Choi H, Ji DJ, Lim CJ. Biomol Ther. 2013; 21:72–78.
2. Tanigichi E, Oshima Y. Agric Biol Chem. 1972; 36:1013–1025.
3. Taniguchi E, Oshima Y. Agric Biol Chem. 1972; 36:1489–1496.
4. Xiao X, Ji Z, Zhang J, Shi B, Wei S, Wu W. Chem Nat Comp. 2013; 49:21–23.
5. Chen C, Zhu H, Zhao D, Deng J, Zhang Y. Helv Chim Acta. 2013; 96:1392–1396.
6. Lee S, Min B, Kho Y. Arch Pharm Res. 2002; 25:652–654.
7. Seo SM, Park IK. Parasitol Res. 2012; 110:1849–1853.
8. Park IK, Shin SC, Kim CS, Lee HJ, Choi WS, Ahn YJ. J Agric Food Chem. 2005; 53:969–972.
9. Singh VK, Mehrotra S, Narayan P, Pandey CM, Agarwal SS. Immunol Res. 2000; 22:1–19.
10. Chesrown SE, Monnier J, Visner G, Nick HS. Biochem Biophys Res Commun. 1994; 200:126–134.
11. Manguro LOA, Lemmen P, Hao P. Rec Nat Prod. 2011; 5:147–157.
12. Schlauer J, Budzianowski J, Kukulczanka K, Ratajczak L. Acta Soc Bot Pol. 2004; 73:9–15.
13. Park HJ, Jung WT, Basnet P, Kadota S, Namba T. J Nat Prod. 1996; 59:1128–1130.
14. Kim MH, Kim JN, Han SN, Kim HK. Immunopharmacol Immunotoxicol. 2015; 37:228–235.
15. Li Q, Dong DD, Huang QP, Li J, Du YY, Li B, Li HQ, Huyan T. Pharm Biol. 2017; 55:799–809.
Full Text Links
  • NPS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr