Infect Chemother.  2010 Feb;42(1):35-38. 10.3947/ic.2010.42.1.35.

Characteristics of CTX-M Type Extended Spectrum beta-lactamase Producing Non-typhoi dal Salmonella Isolates

• Park SH
• Seo YH
• Ahn JY
• Park PH
• Kim KH
• Song YH
• Kim JE

• KMID: 2170278
• DOI: http://doi.org/10.3947/ic.2010.42.1.35

Abstract

BACKGROUND
Extended-spectrum beta-lactamase (ESBL)-producing Salmonella have been increasingly reported worldwide. ESBL-producing Salmonella is of particular concern since children cannot be treated with quinolones. This study was conducted to determine the phenotypic and genetic characteristics of ESBL-producing Salmonella in a tertiary hospital.
MATERIALS AND METHODS
Four clinical ESBL-producing isolates of non-typhoidal Salmonella were collected during 2001 to 2009. Antimicrobial susceptibility was determined by disk diffusion test and VITEK-II system. ESBL production was tested by ESBL phenotypic confirmatory test. TEM, SHV, CTX-M1, CTX-M2, CTX-M8, and CTX-M9 type ESBL genes were detected by PCR amplification, and PCR products were subjected to direct sequencing.
RESULTS
Phenotypic confirmatory test showed that 4 of the 300 non-typhoidal Salmonella isolates were ESBL-producing: 3 S. Enteritidis and 1 S. Typhimurium. All 4 isolates were recovered during the past 1 year period. All 3 S. Enteritidis harbored CTX-M-15, while the S. Typhimurium harbored CTX-M-14. All CTX-M-15-producing S. Enteritidis isolates showed resistance both to cefotaxime and ceftazidime, while the CTX-M-14-producing S. Enteritidis were resistant only to cefotaxime.
CONCLUSIONS
ESBL-producing nontyphoidal Salmonella has emerged recently and the type of ESBL has switched from TEM and SHV to CTX-M.

Keyword

Legionella pneumophila; Diagnosis; Biomarker; cDNA microarray

MeSH Terms

beta-Lactamases
Cefotaxime
Ceftazidime
Child
Diffusion
Humans
Legionella pneumophila
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Quinolones
Salmonella
Cefotaxime
Ceftazidime
Quinolones
beta-Lactamases

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