J Korean Med Sci.  2006 Feb;21(1):1-4. 10.3346/jkms.2006.21.1.1.

Increased Expression of Sodium Transporters in Rats Chronically Inhibited of Nitric Oxide Synthesis

• Kim JS
• Choi KC
• Jeong MH
• Kim SW
• Oh YW
• Lee JU

• KMID: 2157772
• DOI: http://doi.org/10.3346/jkms.2006.21.1.1

Abstract

The present study was done to determine whether endogenous nitric oxide (NO) plays a role in the regulation of sodium transporters in the kidney. Male Sprague-Dawley rats were treated with NG-nitro-L-arginine methyl ester (L-NAME, 100 mg/L drinking water) for 4 weeks. Control rats were supplied with tap water without drugs. Expression of Na, K-ATPase, type 3 Na/H exchanger (NHE3), Na/K/2Cl cotransporter (BSC1), and thiazide-sensitive Na/Cl cotransporter (TSC) proteins was determined in the kidney by Western blot analysis. Catalytic activity of Na,K-ATPase was also determined. The treatment with L-NAME significantly and steadily increased the systemic blood pressure. Total and fractional excretion of urinary sodium decreased significantly, while creatinine clearance remained unaltered. Neither plasma renin activity nor aldosterone concentration was significantly altered. The alpha1 subunit expression and the catalytic activity of Na, K-ATPase were increased in the kidney. The expression of NHE3, BSC1 and TSC was also increased significantly. These results suggest that endogenously-derived NO exerts a tonic inhibitory effect on the expression of sodium transporters, including Na, K-ATPase, NHE3, BSC1, and TSC, in the kidney.

Keyword

Nitric Oxide; Na, K-ATPase; NHE3; BSC1; Sodium-Potassium Chloride Cotransporter 2 Protein; TSC

MeSH Terms

Animals
Blotting, Western
Carrier Proteins/*biosynthesis
Enzyme Inhibitors/pharmacology
Kidney/drug effects/metabolism
Male
NG-Nitroarginine Methyl Ester/*pharmacology
Na(+)-K(+)-Exchanging ATPase/biosynthesis
Nitric Oxide Synthase/*antagonists & inhibitors/metabolism
Rats
Rats, Sprague-Dawley
Receptors, Drug/biosynthesis
Sodium/*metabolism
Sodium Chloride Symporters/biosynthesis
Sodium-Hydrogen Antiporter/biosynthesis
Sodium-Potassium-Chloride Symporters/biosynthesis

Figure

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  Fig. 1 Expression of α1 and β1 subunits of Na,K-ATPase. Representative immunoblots and densitometric data are shown. Symbols are: (■) control and (▒) L-NAME-treatment. Each column represents the mean±SEM of 6 rats. *p<0.05, compared with control.

• 

  Fig. 2 Na,K-ATPase activity. Symbols are: (■) control and (▒) L-NAME-treatment. Each column depicts the mean±SEM of 6 rats. **p<0.01, compared with control.

• 

  Fig. 3 Expression of NHE3. Each column represents the mean±SEM of 6 rats. **p<0.01, compared with control.

• 

  Fig. 4 Expression of BSC-1 and TSC. Each column represents the mean±SEM of 5 rats. *p<0.05, p<0.01; compared with control.

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