Chonnam Med J.  1999 Dec;35(4):553-562.

Endogenous Nitric Oxide Inhibits Na,K-ATPase Activity in the Kidney

• Kim JW
• Kang DG

Abstract

Nitric oxide (NO) has been suggested to play a role in regulating sodium excretion in the kidney. The present study was aimed at investigating the effect of endogenous NO system on Na,K-ATPase activity in the kidney. The enzymatic activity and subunit protein expression of Na,K-ATPase were determined in the kidney in rats treated with NO synthase (NOS) inhibitors, N(G)-nitro-L-arginine methyl ester (L-NAME), for 4 weeks. Following the L-NAME-treatment, renal tissue content and plasma concentrations of NO were decreased, while the expression of ecNOS and iNOS isozymes was not significantly altered. The renal expression of arpha1 and beta1-subunits of Na,K-ATPase did not differ between L-NAME-treated and control rats. However, ouabain-sensitive Na,K-ATPase activities were upregulated following the L-NAME-treatment. These results suggest that endogenous NO plays an inhibitory role in regulating the Na,K-ATPase activity in the kidney.

Keyword

Nitric oxides; Nitric oxide synthase; NG-nitro-L-arginine methyl ester; Na,K-ATPase

MeSH Terms

Animals
Isoenzymes
Kidney*
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase
Nitric Oxide*
Plasma
Rats
Sodium
Isoenzymes
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Sodium