Enhanced Expression of Glyceraldehyde-3-phosphate Dehydrogenase Gene in Human Uterine Cervical Cancers

Kim, K B; Kim, J W; Shin, B Y; Choi, E A; Shin, J W; Park, T C; Lee, J M; Namkoong, S E

Abstract

OBJECTIVE
Tumor cells characteristically exhibit an increased rate of glicolysis. There has been several studies showing an increase of GAPDH gene expression in a variety of human tumors and cancer cell lines. But, in cervical carcinoma, there has not been reported about the level of GAPDH gene expression still now. So, we tried to investigate the GAPDH gene expression patterns in cervical carcinoma tissues and cancer-derived cell lines compard to normal cervical tissues, and the relationships between the level of this gene and conventional clinicopathological parameters were evaluated.
MATERIALS and METHODS
In this study, RNAs from 25 normal exocervical tissues, 35 primary untreated cervical cancer tissues, 2 cervical cancer cell lines (CUMC-3 and CUMC-6), and 2 postnude mice derived cervical cancer cell lines (CUMC-3N and CUMC-6N) were subjected to Northern blot analysis for GAPDH gene expression.
RESULTS
Northern blot analyses revealed that the levels of GAPDH gene expression were markedly elevated in 34 of 35 (97%) cervical carcinoma tissues and all of the four cancer cell lines compared to normal cervical tissues. The level of GAPDH gene expression was highest in rapidly growing postnude mice derived cervical cancer cell lines. And the level of the GAPDH gene expression was not associated with conventional clinicopathological parameters including clinical stage, histological type of the tumor, and degree of differentiation.
CONCLUSION
This result suggests that increased GAPDH gene expression is characteristic of human cervical carcinomas, and antisense oligonucleotide directed against GAPDH mRNA might play a another tool for future gene therapy against cervical carcinoma.