J Korean Ophthalmol Soc.  1999 Jul;40(7):1755-1762.

The Expression of ICAM-1 by Cytokines and the Effect of Dexamethasone on ICAM-1 Expression in Cultured Keratocytes

• Kim HB
• Tchah H

Abstract

Intercellular adhesion molecule-1 (ICAM-1) Is a cell-surface glycoprotein that may regulate leukocyte-endothelial adhesion, leukocyte migration into the tissues, and leukocyte trafficking with target cells during inflammation and immune responses. Expression of ICAM-1 have been observed in diseased cornea, and it has been reported that expression on corneal cells is increased in the presence of pro-inflammatory cytokines. We investigated expression of ICAM-1 by various cytokines on cultured rabbit keratocytes and effect of dexamethasone on cytokine-induced ICAM 1 expression, using an ELISA technique. Cultured rabbit keratocytes were incubated for 24hrs with INF-gamma 10ng/ml, TNF-alpha 10ng/ml IL-1beta 5ng/ml, TGF beta 5ng/ml, with or without 0.1micromiter Dexamethasone. Rabbit keratocytes treated with cytokine or dexamethasone were incubated with antral-ICAM-1 for 15 hours. Expression of ICAM-1 was measured with ELISA technique. As a result, expression of ICAM-1 was increased in rabbit keratocytes stimulated with INF-gamma, TNF-alpha, TGF-beta, not IL-1beta, and dexamethasone inhibited expression of ICAM-1 in cells stimulated with INF-gamma, TNF-alpha. This results are helpful to understand the role of ICAM-1 in the pathophysiology of inflammatory corneal diseases and the action mechanism of glucocorticosteroids. Further study about expression of ICAM-1 and their regulation & modulation may lead to new therapies in treating inflammatory corneal diseases.

Keyword

ICAM-1; Keratocyte; Cytokines; Dexamethasone; Immunocytochemical study

MeSH Terms

Cornea
Corneal Diseases
Cytokines*
Dexamethasone*
Enzyme-Linked Immunosorbent Assay
Glycoproteins
Inflammation
Intercellular Adhesion Molecule-1*
Leukocytes
Transforming Growth Factor beta
Tumor Necrosis Factor-alpha
Cytokines
Dexamethasone
Glycoproteins
Intercellular Adhesion Molecule-1
Transforming Growth Factor beta
Tumor Necrosis Factor-alpha