J Korean Ophthalmol Soc.  1990 Feb;31(2):155-159.

The Effect of a Collagen Shield on Concentrations of Tobramycin in Corneas

• Baik JM
• Kim KS
• Oh JS

Abstract

Collagen shields made of porcine scleral collagen were immersed in a solution containing tobramycin sulfate(40 mg/ml) for five minutes, then applied to rabbit corneas. One, four and eight hours after application, the corneas were assayed for antibiotics by agar diffusion bioassay. We divided study groups as 4 groups, that is, collagen shield, subconjunctival, therapeutic soft contact lens and topical group, and compared the corneal concentradon of tobramycin in each group. Shields immersed in 40 mg/ml tobramycin produced significantly higher concentrations of tobramycin in the cornea at one and four hours than those of the other groups. Tobramycin concentrations in cornea eight hours after application became lower than those of one and four hours after application but, higher than minimal inhibitory concentration of Pseudomonas aeruginosa. Collagen shield containing antibiotics can serve as a vehicle for drug delivery and may prove superior to current methods for the initial treatment of bacterial keratitis, and preoperative and postoperative antibiotic prophylaxis.

Keyword

agar diffusion bioassay; collagen shield; corneal concentration; tobramycin

MeSH Terms

Agar
Anti-Bacterial Agents
Antibiotic Prophylaxis
Biological Assay
Collagen*
Contact Lenses, Hydrophilic
Cornea*
Diffusion
Keratitis
Pseudomonas aeruginosa
Tobramycin*
Agar
Anti-Bacterial Agents
Collagen
Tobramycin