J Korean Orthop Assoc.  2008 Apr;43(2):171-180. 10.4055/jkoa.2008.43.2.171.

Gene Expression Profile Analysis by cDNA Array in the Subacromial Bursa of Patients with Rotator Cuff Disease

• Kim YS
• Kim JM
• Jung HW
• Bigliani L
• Blaine TA

• KMID: 2106436
• DOI: http://doi.org/10.4055/jkoa.2008.43.2.171

Abstract

PURPOSE: To examine the expression pattern of inflammatory cytokines/receptors in the subacromial bursa of patients with rotator cuff disease using a cDNA(Complement DNA) Array technique.
MATERIALS AND METHODS
Twenty two human subacromial bursal specimens were obtained intraoperatively from patients during shoulder surgery (18 bursitis, 4 normal bursa). The RNA was isolated from the bursal tissues and the presence of gene expression was analyzed using a cDNA Array technique. The statistical differences between bursitis and the normal bursa specimens were determined using a Mann Whitney U test and Student's t-test.
RESULTS
cDNA Array analysis revealed a significant increase in the expression of several cytokine genes and their receptors in patients with subacromial bursitis compared with the controls (p<0.05). These cytokines included the interleukins (IL-1, 6, 12, 13, 15, 16, 17) and their receptors, lymphotoxin, small inducible cytokines, chemokine receptor (CCR 4, 6, 7) and stromal cell derived factor-1 (SDF-1).
CONCLUSION
This study demonstrated a significant increase in many inflammatory cytokines in the subacromial bursa of patients with rotator cuff disease. This suggests that there is an active inflammatory reaction at the subacromial bursa in rotator cuff disease.

Keyword

Shoulder; Rotator cuff disease; Subacromial bursitis; Inflammatory cytokines/receptors; cDNA array

MeSH Terms

Bursitis
Cytokines
DNA, Complementary
Gene Expression
Humans
Interleukins
Lymphotoxin-alpha
Oligonucleotide Array Sequence Analysis
RNA
Rotator Cuff
Shoulder
Stromal Cells
Transcriptome
Cytokines
DNA, Complementary
Interleukins
Lymphotoxin-alpha
RNA

Figure

• 

  Fig. 1 X-ray films of the chemiluminescent detection membrane for human inflammatory cytokine/receptor gene array*. The expression of IL-1A , IL-6, SDF-1 and control (GAPD) is indicated (arrow). For example, IL-1A, IL-6 and SDF-1 are strongly expressed on the membrane of a patient with rotator cuff disease, but there is no expression observed on the control membrane. The GAPDH gene was used as the control gene, which should have the same expression on both membranes. GAPD, Glyceraldehyde-3-phosphate dehydrogenase.

• 

  Fig. 2 Average relative intensity of the expression of the interleukin/receptor gene in the subacromial bursa specimens. The gene expression of interleukins and receptors was significantly higher in the rotator cuff disease group than the control group (p<0.05).

• 

  Fig. 3 Average relative intensity of the expression of the inflammatory cytokine/receptor gene in the subacromial bursa specimens. The gene expression of the inflammatory cytokines and receptors was significantly higher in the rotator cuff disease group than the control group (p<0.05).

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