J Korean Orthop Assoc.  2008 Apr;43(2):171-180. 10.4055/jkoa.2008.43.2.171.

Gene Expression Profile Analysis by cDNA Array in the Subacromial Bursa of Patients with Rotator Cuff Disease

Affiliations
  • 1Department of Orthopedic Surgery, Kangnam St. Mary's Hospital, The Catholic University of Korea College of Medicine, Seoul, Korea. osjmk@korea.com
  • 2Center for Shoulder, Elbow and Sports Medicine, Columbia University College of Physicians and Surgeons, New York, USA.

Abstract

PURPOSE: To examine the expression pattern of inflammatory cytokines/receptors in the subacromial bursa of patients with rotator cuff disease using a cDNA(Complement DNA) Array technique.
MATERIALS AND METHODS
Twenty two human subacromial bursal specimens were obtained intraoperatively from patients during shoulder surgery (18 bursitis, 4 normal bursa). The RNA was isolated from the bursal tissues and the presence of gene expression was analyzed using a cDNA Array technique. The statistical differences between bursitis and the normal bursa specimens were determined using a Mann Whitney U test and Student's t-test.
RESULTS
cDNA Array analysis revealed a significant increase in the expression of several cytokine genes and their receptors in patients with subacromial bursitis compared with the controls (p<0.05). These cytokines included the interleukins (IL-1, 6, 12, 13, 15, 16, 17) and their receptors, lymphotoxin, small inducible cytokines, chemokine receptor (CCR 4, 6, 7) and stromal cell derived factor-1 (SDF-1).
CONCLUSION
This study demonstrated a significant increase in many inflammatory cytokines in the subacromial bursa of patients with rotator cuff disease. This suggests that there is an active inflammatory reaction at the subacromial bursa in rotator cuff disease.

Keyword

Shoulder; Rotator cuff disease; Subacromial bursitis; Inflammatory cytokines/receptors; cDNA array

MeSH Terms

Bursitis
Cytokines
DNA, Complementary
Gene Expression
Humans
Interleukins
Lymphotoxin-alpha
Oligonucleotide Array Sequence Analysis
RNA
Rotator Cuff
Shoulder
Stromal Cells
Transcriptome
Cytokines
DNA, Complementary
Interleukins
Lymphotoxin-alpha
RNA
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