J Korean Surg Soc.  2010 Dec;79(6):421-427. 10.4174/jkss.2010.79.6.421.

The Wound Healing Effect of a Silk Fibroin Film on Cutaneous Burn of Hairless Mice

• Choi YH
• Kim MG
• Ahn DH
• Hong SH
• Lee JY
• Kim HS
• Kim HC
• Nam SY
• Yim G

• KMID: 2096596
• DOI: http://doi.org/10.4174/jkss.2010.79.6.421

Abstract

PURPOSE
The aim of this study was to examine the effects of silk fibroin film on wound healing of cutaneous burn in hairless mice by using microscopic findings and stem cell markers (nestin, cytokeratin 15) and ki-67 proliferation marker.
METHODS
Each mouse received two burns at the dorsal area by applying a metal rod heated with boiling water. Burn wound sites were dressed with Silk Fibroin Film and duoderm (SF group), Aquacel hydrofiber and duoderm (AC group) and duoderm only (Control group). All groups were covered externally with duodermas adhesive bands. Those mice were sacrificed at zero, two, seven, fourteen and twenty one days after burn. Histological findings and immunohistochemical staining for stem cell markers were observed.
RESULTS
In SF group, inflammatory cell infiltration, formation of granulation tissue and inflammatory foci are greater than in AC and control group. Those factors appear to enhance mesenchymal stem cell markers such as nestin. Finally mesenchymal tissue regeneration was enhanced. In addition, the length of ki-67 expressed re-generating epithelium, which appeared to be associated with epithelial regeneration, was the longest in SF group.
CONCLUSION
The results show that the wound healing effect of SF is the best among other treatment materials including AC in the experimental group and duoderm in the control group through mesenchymal regeneration and epithelial regeneration which are essential factors for wound healing.

Keyword

Hairless mice; Silk fibroin film; Burn; Wound healing; Stem cell

MeSH Terms

Adhesives
Animals
Bandages, Hydrocolloid
Burns
Carboxymethylcellulose Sodium
Epithelium
Fibroins
Granulation Tissue
Hot Temperature
Intermediate Filament Proteins
Keratins
Mesenchymal Stromal Cells
Mice
Mice, Hairless
Nerve Tissue Proteins
Regeneration
Silk
Stem Cells
Water
Wound Healing
Adhesives
Carboxymethylcellulose Sodium
Fibroins
Intermediate Filament Proteins
Keratins
Nerve Tissue Proteins
Silk
Water

Figure

• 

  Fig. 1 Immunohistochemical expression of nestin in granulation tissue 7 days after burn. The long and thin arrow indicates necrotic tissue after burn. Nestin presents in the capillaries and peripheral nerve bundles (short and thick arrow) in the granulation tissue under the necrotic tissue (×40).

• 

  Fig. 2 Immunohistochemical expression of keratin 15 in regenerating epithelium 2 days after burn. A shows the distal part of regenerating epithelium without keratin 15 expression. B shows the proximal part of regenerating epithelium with keratin 15 expression of superficial layer. C shows the normal epidermis with keratin 15 expression of the full thickness skin except basal layer (×40, Inset: ×100).

• 

  Fig. 3 Immunohistochemical expression of Ki-67 in regenerating epithelium on 2 days after burn. A shows the distal part of regenerating epithelium with sparse Ki-67 expression. B shows the proximal part of regenerating epithelium with Ki-67 expression of 2~3 basal layers. C shows the normal epidermis with Ki-67 expression of basal layer (×40, Inset: ×100).

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