Korean J Dermatol.
2000 Mar;38(3):305-313.
Effects of Topical Application of Halofuginone on Wound Healing
Abstract
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BACKGROUND: During normal wound healing the formation of scars and fibrous tissue occurs,
which consists largely of collagen fibril, but excessive fibrosis and scar formation become
clinical problems. Collagen remodelling during scar formation is dependent on both continued
collagen synthesis and collagen catabolism. Halofuginone, a plant alkaloid, is known to
inhibit collagen type I synthesis at the transcriptional level.
OBJECTIVE
The purposes of this study were to investigate the effects of topical
application of halofuginone on the healing of wounds.
METHOD: Topical solutions containing halofuginone of variable concentrations were applied
on the full-thickness excisional wounds of hairless mice and 0.1% halofuginone ointments
applied on the suture site of rats and the normal skin of hairless mice daily. In addition,
we performed a one-time intradermal injection of 0.1% halofuginone solution on the normal
skin of the hairless mice. We examined the collagen content of the skin of hairless mice
and rats treated with halofuginone solutions and ointments during the healing process by
performing hematoxylin-eosin and Masson's trichrome stains. We assessed, from time to time,
the change in the full-thickness excisional wound size of hairless mice treated with
halofuginone solutions of variable concentrations during the healing process and observed
clinically the healing process of hairless mice with the full-thickness excisional wound.
RESULT: 1. The wound size after daily application of 0.001% and 0.1% halofuginone solutions
on the full-thickness excisional wounds of hairless mice decreased more slowly in comparison
with the control group (p < 0.05).