J Periodontal Implant Sci.  2011 Aug;41(4):192-200. 10.5051/jpis.2011.41.4.192.

Gene expression profile in mesenchymal stem cells derived from dental tissues and bone marrow

• Kim SH
• Kim YS
• Lee SY
• Kim KH
• Lee YM
• Kim WK
• Lee YK

• KMID: 2094719
• DOI: http://doi.org/10.5051/jpis.2011.41.4.192

Abstract

PURPOSE
The aim of this study is to compare the gene expression profile in mesenchymal stem cells derived from dental tissues and bone marrow for characterization of dental stem cells.
METHODS
We employed GeneChip analysis to the expression levels of approximately 32,321 kinds of transcripts in 5 samples of bone-marrow-derived mesenchymal stem cells (BMSCs) (n=1), periodontal ligament stem cells (PDLSCs) (n=2), and dental pulp stem cells (DPSCs) (n=2). Each cell was sorted by a FACS Vantage Sorter using immunocytochemical staining of the early mesenchymal stem cell surface marker STRO-1 before the microarray analysis.
RESULTS
We identified 379 up-regulated and 133 down-regulated transcripts in BMSCs, 68 up-regulated and 64 down-regulated transcripts in PDLSCs, and 218 up-regulated and 231 down-regulated transcripts in DPSCs. In addition, anatomical structure development and anatomical structure morphogenesis gene ontology (GO) terms were over-represented in all three different mesenchymal stem cells and GO terms related to blood vessels, and neurons were over-represented only in DPSCs.
CONCLUSIONS
This study demonstrated the genome-wide gene expression patterns of STRO-1+ mesenchymal stem cells derived from dental tissues and bone marrow. The differences among the expression profiles of BMSCs, PDLSCs, and DPSCs were shown, and 999 candidate genes were found to be definitely up- or down-regulated. In addition, GOstat analyses of regulated gene products provided over-represented GO classes. These data provide a first step for discovering molecules key to the characteristics of dental stem cells.

Keyword

Adult stem cells; Gene expression profiling; Mesenchymal stem cells; Microarray analysis; mRNA

MeSH Terms

Adult Stem Cells
Blood Vessels
Bone Marrow
Dental Pulp
Gene Expression
Gene Expression Profiling
Mesenchymal Stromal Cells
Microarray Analysis
Morphogenesis
Neurons
Periodontal Ligament
Resin Cements
RNA, Messenger
Stem Cells
Transcriptome
RNA, Messenger
Resin Cements

Figure

• 

  Figure 1 Isolation of STRO-1+ cells from each heterogeneous population using a FACS sorter machine. The STRO-1 marker identifies a subgroup of STRO-1+ mesenchymal stem cells: PDLSCs=0.96%, DPSCs=0.81%, BMSCs=0.73%. PDLSCs: periodontal ligament stem cells, DPSCs: dental pulp stem cells, BMSCs: bone-marrow-derived mesenchymal stem cells.

• 

  Figure 2 Heatmap comparing 53 genes most differentially expressed among the groups. Each row corresponds to a gene, with the columns corresponding to expression levels in different samples. Color varies from red, for the lowest levels of expression, to green for the highest level of expression.

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