A Study on Tests of Skin Safety and Inhibition of Atopic Dermatitis Using a StoneTouch(R) Infrared Scanner in a Mouse Model

Lim, Yun Young; Kim, Hyeong Mi; Jang, Woo Sun; Seo, Soo Hong; Ahn, Hyo Hyun; Kim, Myeung Nam; Kim, Beom Joon

Abstract

BACKGROUND
Atopic dermatitis is a chronic inflammatory skin disease. It is caused by immunological abnormalities, abnormalities of the skin barrier, environmental factors and genetic factors. Atopic dermatitis destroys the skin barrier and passes through the skin, triggering an immune response. To treat atopic dermatitis, we anticipate use of hypoallergenic cures to hydrate skin that has been dried by destruction of the skin barrier.
OBJECTIVE
We did a preclinical trial to identify inhibitory effects of the StoneTouch(R) infrared scanner on atopic dermatitis. We conducted skin safety tests, comparing the use of infrared energy to drug treatment. We then confirmed the effects of the StoneTouch(R) infrared scanner through animal tests using Nc/Nga mice as a model of atopic dermatitis in order to identify any inhibition of the immune response in atopic dermatitis.
METHODS
We irradiated Nc/Nga mice using a StoneTouch(R) infrared scanner under a variety of conditions. During skin safety tests of the StoneTouch(R) infrared scanner on hairless mice, we assessed immune response and burn risk in irradiated mouse skin. We identified any inhibitory effects on atopic dermatitis using Dermoscope assessments, measurements of transepidermal water loss (TEWL) and IgE levels, measurements of pro-inflammatory cytokines, H&E staining and immunofluorescence staining (IF) of substance P and CGRP as neurotransmitters on the backs and ears of Nc/Nga mice irradiated by the StoneTouch(R) infrared scanner.
RESULTS
We did not observe any skin abnormalities after using the StoneTouch(R) infrared scanner on Nc/Nga mice. We confirmed the inhibitory effect of the StoneTouch(R) infrared scanner irradiation on atopic dermatitis. We found that irradiated epidermis was thinner than that of the epidermis in Nc/Nga mice in which atopic dermatitis was induced. We observed no significant between groups differences in expression level of substance P. The expression of CGRP in mice with atopic dermatitis was decreased, but, the increased irradiation led to greater expression of CGRP in irradiated skin.
CONCLUSION
The StoneTouch(R) infrared scanner does not as a function of irradiation dosage. It inhibits the development of atopic dermatitis.