Korean J Phys Anthropol.  1999 Jun;12(1):169-174.

Up-regulation of Human nm23-H2/NDP Kinase B in Early Phase of Regenerating Transgenic Mouse Hepatocyte

• Lee IH

Abstract

The kinetics and subcellular localization of nm23-M1 and -M2 was reported in in vivo regenerating mouse liver cells after partial hepatectomy (Lee et al. 1997a). On the human nm23-H2 transgenic mice, the kinetics of murine types of nm23 and mitotic index were very similar to results from ordinary mice B6, whereas the kinetics of human type of nm23 was responded strongly and shortly in early phase of regeneration. In subcellular study, all fractions of nm23-H2 protein at 6hr after partial hepatectomy were increased to very high level and decreased soon day 1. These studies suggest that exogenous DNA sequence nm23-H2 expressed immediately in early phase of hepatocyte regeneration, and in short term duration comparing with native murine type of nm23. This analysis might include that another cellular cofactor(s) is(are) necessary for human type nm23 expression in regenerating mouse hepatocyte in playing a role as a transcription factor, or that is not associated with any cellular cofactors in organ crisis.

Keyword

nm23/NDP kinase; Transgenic mice; Regeneration; Mouse; Hepatocyte; Transcription effect

MeSH Terms

Animals
Base Sequence
Hepatectomy
Hepatocytes*
Humans*
Kinetics
Liver
Mice
Mice, Transgenic*
Mitotic Index
Phosphotransferases*
Regeneration
Transcription Factors
Up-Regulation*
Phosphotransferases
Transcription Factors