J Bacteriol Virol.  2007 Jun;37(2):61-68. 10.4167/jbv.2007.37.2.61.

Antimicrobial Activity of Extract and Fractions from Drynaria fortunei Against Oral Bacteria

  • 1Department of Dental Hygiene, Ulsan College, San 160-1 Hwajeong-Dong, Dong-Gu, Ulsan, 682-715, Korea. ekjung@mail.uc.ac.kr


One of the traditional Korean medicine, Drynaria fortunei (D. fortunei) is one of candidates known to be effective for the treatment of inflammation, hyperlipemia, arteriosclerosis, rheumatism, and gynecological diseases such as osteoporosis and bone resorption. The present study investigated the antimicrobial activity of methanol (MeOH) extract and n-butanol (n-BuOH), chloroform (CHCl3), and ethyl acetate (EtOAc) fractions of D. fortunei against oral bacteria. The n-BuOH and CHCl3 fractions (MICs, 0.0078 to 0.3125 mg/ml; MBCs, 0.019 to 0.625 mg/ml) were demonstrated as strong antibacterial activity than the MeOH extract and EtOAc fraction. The combination effects of n-BuOH fraction with ampicillin or gentamicin were synergistic against some oral bacteria. We suggest that D. fortunei could be employed as a natural antibacterial agent in oral care products.


Drynaria fortunei; Antibacterial activity; Minimum inhibitory concentrations/minimum bactericidal concentrations

MeSH Terms

Bone Resorption
Rheumatic Diseases


  • Figure 1. Time-kill curves of MCs of n-BuOH fraction alone and its combination with MICs of ampicillin or gentamicin against. S. mutans, S. sanguinis, S. sobrinus, S. anginosus, S. criceti, and S. ratti. Bacteria were incubated with n-BuOH fraction along (), n-BuOH fraction with ampicillin (❍), and n-BuOH fraction with gentamicin (▾) over time. Data points are the mean values ± S.E.M. of four experiments. CFU, colony-forming units.

  • Figure 2. Time-kill curves of MICs of n-BuOH fraction alone and its combination with MICs of ampicillin or gentamicin against S. gordonii, A. actinomycetemcomitans, F. nucleatum, P. intermedia, and P. gingivalis. Bacteria were incubated with n-BuOH fraction along (), n-BuOH fraction with ampicillin (❍), and n-BuOH fraction with gentamicin (▾) over time. Data points are the mean values ± S.E.M. of four experiments. CFU, colony-forming units.



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