J Korean Acad Oral Health.  2022 Dec;46(4):174-178. 10.11149/jkaoh.2022.46.4.174.

Antimicrobial effects of green tea extract-containing dentifrice

Affiliations
  • 1Department of Oral Health, College of Health Science, Dankook University, Cheonan, Korea
  • 2Department of Preventive Dentistry, College of Dentistry, Dankook University, Cheonan, Korea

Abstract


Objectives
This study aimed to evaluate the antibacterial activity of green tea extract containing dentifrice on oral disease-causing bacteria.
Methods
The antibacterial activity of green tea extract containing dentifrice was examined on the gram-positive bacteria Actinomyces israelii, Enterococcus faecalis, Filifactor alocis, Streptococcus mutans, and gram-negative bacteria Fusobacterium nucleatum, Porphyromonas gingivalis. A. israelii, E. faecalis, F. alocis, S. mutans, F. nucleatum, and P. gingivalis were cultured with green tea extract containing dentifrice or control dentifrice; they were then incubated at 37°C under anaerobic conditions and cultured. Following incubation, a microplate reader was used to measure the absorbance and observe the number of bacteria. Statistical significance tests were conducted using the Mann-Whitney test and SPSS 24.0.
Results
Green tea containing dentifrice has antibacterial activity against A. israelii, E. faecalis, F.alocis, S. mutans, F. nucleatum, and P. gingivalis. Furthermore, green tea containing dentifrice killed more than 99.99% of 4 strains of gram-positive bacteria and 2 strains of gram-negative bacteria.
Conclusions
The detergent with green tea extract is significantly reduced the bacteria applied in this experiment; dentifrice has a bactericidal and antibacterial effect against oral bacteria. Therefore, the detergent with green tea extract is thought to be helpful in preventing dental caries and periodontal disease.

Keyword

Antimicrobial activity; Dentifrice; Green tea extract; Oral bacteria

Figure

  • Fig. 1 Antibacterial activity test of the experimental dentifrice against A. israelii. A. israelii was cultivated with control or experimental in the various concentration. The growth of A. israelii was measured by spectrophotometer at 660 nm of wavelength. *Statistically significant compared to control group (P<0.05).

  • Fig. 2 Antibacterial activity test of the experimental dentifrice against E. faecalis. E. faecalis was cultivated with control or experimental in the various concentration. The growth of E. faecalis was measured by spectrophotometer at 660 nm of wavelength. *Statistically significant compared to control group (P<0.05).

  • Fig. 3 Antibacterial activity test of the experimental dentifrice against F. alocis. F. alocis was cultivated with control or experimental in the various concentration. The growth of F. alocis was measured by spectrophotometer at 660 nm of wavelength. *Statistically significant compared to control group (P<0.05).

  • Fig. 4 Antibacterial activity test of the experimental dentifrice against F. nucleatum. F. nucleatum was cultivated with control or experimental in the various concentration. The growth of F. nucleatum was measured by spectrophotometer at 660 nm of wavelength. *Statistically significant compared to control group (P<0.05).

  • Fig. 5 Antibacterial activity test of the experimental dentifrice against P. gingivalis. P. gingivalis was cultivated with control or experimental in the various concentration. The growth of P. gingivalis was measured by spectrophotometer at 660 nm of wavelength. *Statistically significant compared to control group (P<0.05).

  • Fig. 6 Antibacterial activity test of the experimental dentifrice against S. mutans. S. mutans was cultivated with control or experimental in the various concentration. The growth of S. mutans was measured by spectrophotometer at 660 nm of wavelength. *Statistically significant compared to control group (P<0.05).


Reference

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