Tuberc Respir Dis.  1998 Aug;45(4):697-704. 10.4046/trd.1998.45.4.697.

Eotaxin mRNA Expression in Bronchial Mucosa of Patients with Asthma

• In KH
• Cho JY
• Kang SY
• Lee SY
• Shim JJ
• Kang KH
• Yoo SH
• Na YS
• Kim HG

• KMID: 2050085
• DOI: http://doi.org/10.4046/trd.1998.45.4.697

Abstract

BACKGROUND: Asthma is a chronic inflammatory disease of the airways characterized by a marked infiltration of ecsinophils in the bronchial mucosa. Asthmatic bronchial muosa produces many factors described as king chernotaetic for inflammatory cells. IL-5, RANTES, and MCP-1 alpha are the chemotactic factors for eosinophils, but their roles are controversiaL Recently eotaxin that is a potent eosinophil chernoattracttnt cytokine was detected in a guinea-pig model of allergic airway inflammation, and human eotaxin was cloned. Eotaxin is a specific chemoattractant for eosinophils, but its role in asthma is not confirmed. We examined the in vivo expression of a,taxin in bronchi of asthmatic patients.
METHODS
11 asthmatics and 2 normal controls were enrolled. All subjects were underwent brcnchcscopy with bronchial biopsies in 2nd or 3rd carina. RNA extraction from biopsy samples was done by acid-guanidium method. Semi-quantitaive RT-PCR was done for evaluation of eotaxin mRNA expression. The extent of eosinophil infiltrartion was evaluated by counting the eosinophils in submucosa in HPF of microscope.
RESULTS
Eotaxin mRNA expressed in symptomatic, uncontrolled asthma. Steroid inhibited expression of eotaxin mRNA in asthma. Expression of eotaxin mRNA correlated with eosinohil infiltration in bronchial tissues. CONCLUISON: Expression of eotaxin mRNA increases in uncontrolled asthma and eotaxin is involved in the recruitment of eosinophils.

Keyword

Eotaxin; Asthma; Bronchial mucosa

MeSH Terms

Asthma*
Biopsy
Bronchi
Chemokine CCL5
Chemotactic Factors
Clone Cells
Eosinophils
Humans
Inflammation
Interleukin-5
Mucous Membrane*
RNA
RNA, Messenger*
Chemokine CCL5
Chemotactic Factors
Interleukin-5
RNA
RNA, Messenger