Korean J Vet Res.  2015 Sep;55(3):181-184. 10.14405/kjvr.2015.55.3.181.

Expression analysis of ciliary rootlet coiled coil protein mRNA during Xenopus development

• Rahman MM
• Kim IS
• Ahn DC
• Cho HS
• Kim WI
• Kim B
• Shin GW
• Kwon J
• Akanda MR
• Park BY

• KMID: 2049853
• DOI: http://doi.org/10.14405/kjvr.2015.55.3.181

Abstract

Ciliary rootlet coiled coil protein (CROCC), the structural component that originates from the basal body at the proximal end of the ciliary rootlet, plays a crucial role in maintaining the cellular integrity of ciliated cells. In the current study, we cloned Xenopus CROCC and performed the expression analysis. The amino acid sequence of Xenopus laevis was related to those of Drosophila, cow, goat, horse, chicken, mouse and human. Reverse transcription polymerase chain reaction analysis revealed that CROCC mRNA encoding a coiled coil protein was present maternally, as well as throughout early development. In situ hybridization indicated that CROCC mRNA occurred in the animal pole of embryo during gastrulation and subsequently in the presumptive neuroectoderm at the end of gastrulation. At tailbud stages, CROCC mRNA expression was localized in the anterior roof plate of the developing brain, pharyngeal epithelium connected to gills, esophagus, olfactory placode, intestine and nephrostomes of the pronephric kidney. Our study suggests that CROCC may be responsible for control of the development of various ciliated organs.

Keyword

ciliary rootlet coiled coil protein; esophagus; nephrostomes; pharyngeal epithelium; Xenopus

MeSH Terms

Amino Acid Sequence
Animals
Basal Bodies
Brain
Chickens
Clone Cells
Drosophila
Embryonic Structures
Epithelium
Esophagus
Gastrulation
Gills
Goats
Horses
Humans
In Situ Hybridization
Intestines
Kidney
Mice
Neural Plate
Polymerase Chain Reaction
Reverse Transcription
RNA, Messenger*
Xenopus laevis
Xenopus*
RNA, Messenger