Korean J Oral Maxillofac Radiol.  2003 Mar;33(1):51-57.

The effects of irradiation on the mRNA expression of type I collagen and alkaline phosphatase in the MC3T3-E1 osteoblastic cell line

• Choi SY
• Koh KJ

Abstract

PURPOSE
To investigate the effects of irradiation on the phenotypic expression of the MC3T3-E1 osteoblastic cell line, particularly an the expression of type I collagen and alkaline phosphatase mRNA. MATERIALS AND METHODS: Cells were irradiated with a single dose of 0.5, 1, 2, 4, and 8 Gy at a dose rate of 5.38 Gy/min using a cesium 137 irradiator. The specimens were then harvested and RNA extraction was carried out at 1 and 3 days after irradiation. The extracted RNA strands were reverse-transcribed and the resulting cDNA fragments were amplified by PCR. RESULTS: The irradiated cells demonstrated a dose-dependent increase in type I collagen mRNA expression relative to the control group, with a maximum level of type I collagen mRNA expression occurring at 8 Gy. The degree of type I collagen mRNA expression increased significantly at 1 day after irradiation, but little differences were found between the control group and at the 3rd day. The amount of alkaline phosphatase mRNA expression increased significantly at 1 and 3 days after irradiation in the 1 Gy exposed group compared with the control group. CONCLUSION: The amount of type I collagen and alkaline phosphatase mRNA expression increased significantly 1 day after irradiation when compared with the control group.

Keyword

irradiation; collagen type I; alkaline phosphatase; osteoblasts

MeSH Terms

Alkaline Phosphatase*
Cell Line*
Cesium
Collagen Type I*
DNA, Complementary
Osteoblasts*
Polymerase Chain Reaction
RNA
RNA, Messenger*
Alkaline Phosphatase
Cesium
Collagen Type I
DNA, Complementary
RNA
RNA, Messenger