J Korean Assoc Maxillofac Plast Reconstr Surg.  2005 Sep;27(5):424-430.

Gene expression after the application of the fluid-induced shear stress on the gingival fibroblast

• Jeong MH
• Choi JY
• Chae CH
• Kim SG
• Nahm DS

Abstract

The oral cavity is humid environment mainly due to the continuous salivary flow. The reaction of oral mucosa to fluid flow is important for homeostasis and pathogenesis. The objective of this study is the screening the change of gene expression after the application of fluid induced shear stress (FISS) on the gingival fibroblast using cDNA microarray assay. The immortalized human gingival fibroblasts were grown and FISS was applied using a cone viscometer at a rotational velocity of 40 rpm, respectively for periods of 2 and 4 hours. The synthesis of cDNA was done from the extracted total RNA and cDNA microarray assay was done subsequently. The genes that showed over 1.6 in the Cy3/Cy5 or the Cy5/Cy3 value were regarded as genes influenced significantly by the FISS application (/M/>0.7). The "RUNX-1" was increased its expression in 2 hours group and "RUN and SH3 domain containing 1" was increased its expression in 4 hours group. The "CC020415", "cyclin L1", "interferon regulatory factor1", "early growth response 1", "immediate early response 2", and "immediate early response 3" genes were increased their expression in 2 and 4 hours after FISS application. In conclusion, we could find many genes that were probably related to the FISS application. Interestingly, most of them were placed in similar molecular pathways and these findings improve the reliability of chip data and usefulness in overall screening. From this experiment, we could find many items for further study and it will make improvement in the understanding of intracellular events in response to FISS.

Keyword

Gingival fibroblast; cDNA microarray; Fluid induced shear stress

MeSH Terms

DNA, Complementary
Fibroblasts*
Gene Expression*
Homeostasis
Humans
Mass Screening
Mouth
Mouth Mucosa
Oligonucleotide Array Sequence Analysis
RNA
src Homology Domains
DNA, Complementary
RNA