J Korean Acad Conserv Dent.  2004 Sep;29(5):479-484. 10.5395/JKACD.2004.29.5.479.

The effect of Treponema denticola immunoinhibitory protein on cytokine expression in T cells

• Lee SY
• Shon WJ
• Lee W
• Baek SH
• Bae KS
• Lim S

• KMID: 1987128
• DOI: http://doi.org/10.5395/JKACD.2004.29.5.479

Abstract

Immunoinhibitory protein extracted from sonicated Treponema denticola have been shown to suppress cell cycle progression of human lymphocytes. To study in detail about the effect of this microorganism on the function of lymphocytes, we investigated the levels of Interleukin-2 (IL-2) and Interleukin-4 (IL-4) production by T lymphocytes before and after the addition of 12.5 microg/ml T. denticola sonicated extracts. In this study, levels of IL-2 and IL-4 produced from T cells pretreated with sonicated extracts were evaluated by using the quantitative sandwich enzyme immunoassay technique. In response to phytohemagglutinin (PHA) stimulation, T cell produced increased levels of IL-2 and IL-4. However, the expressions of both cytokines were significantly inhibited when PHA activated-T cells were pre-exposed to sonicated T. denticola extracts (p < 0.05). These findings suggest that the T. denticola sonicated extracts induced-immunosuppression in Th1 and Th2 cell functions could be a part of the pathogenic mechanism of the endodontic failure associated with this microorganism.

Keyword

Immunoinhibitory protein; T. denticola; Sonicated extracts; Interleukin-2; Interleukin-4

MeSH Terms

Cell Cycle
Cytokines
Humans
Immunoenzyme Techniques
Interleukin-2
Interleukin-4
Lymphocytes
T-Lymphocytes*
Th2 Cells
Treponema denticola*
Treponema*
Cytokines
Interleukin-2
Interleukin-4

Figure

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  Figure 1 Expression of IL-2 in each group. The data represent mean values of three experiments done in duplicate.

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  Figure 2 Expression of IL-4 in each group. The data represent mean values of three experiments done in duplicate.

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