J Korean Acad Conserv Dent.  2002 Sep;27(5):473-478. 10.5395/JKACD.2002.27.5.473.

Sonicated extract of Treponema denticola impairs the lymphocyte proliferation

• Lee WC
• Shenker BJ

• KMID: 1987303
• DOI: http://doi.org/10.5395/JKACD.2002.27.5.473

Abstract

No abstract available.

MeSH Terms

Lymphocytes
Treponema
Treponema denticola

Figure

• 

  Fig. 1 Chromatography of soluble extracts of Treponema denticola LL2513.

• 

  Fig. 2 Effects of SIP on lymphocyte proliferation. HPBMC were incubated with various amounts SIP for 30 min and PHA (1.0µg/ml) was added. The cells were then incubated for 72 hr. 3H-TdR was added for the last 4 hr. Data was presented as a percent inhibition of 3H-TdR incorporation. Results represent the mean ± SD of 5 experiments

• 

  Fig. 3 Cell cycle analysis using single PI staining. HPBMC were incubated with medium (A), PHA (B), PHA and 0.5µg of SIP per ml (C), 0.25µg of SIP per ml (D) for 72 h, and the cells were stained as described in materials and methods. Results are the mean of replicate cultures and are representative of three experiments.

Reference

1. Listgarten MA. Genco RJ, Mergenhangen SE, editors. Colonization of subgingival areas by motile rods and spirochetes: clinical implication. Host-Parasite Interaction in Periodontal Diseases. 1982. Washington D.C: American Society of Microbiology;112.

2. Thilo B, Baehni P, Holz J, Baume LJ. Distribution des bacteries dans les parties coronaire et apicale de dents a pulpe necrosee. SSO Schweiz Monatsschr Zahnheilkd. 1983. 93:335–350.

3. Nair PNR. Light and electron microscopic studies of root canal flora and periapical lesions. J Endod. 1987. 13:29–39.

4. Siqueira JF, Rocas IN, Favieri A, Santos KRN. Detection of Treponema denticola in endodontics infections by 16S rRNA gene detected polymerase chain reaction. Oral Microbiol Immunol. 2000. 15:335–337.

5. Siqueira JF, Rocas IN, Oliveira JCM, Santos KRN. Detection of putative oral pathogens in acute periradicular abscesses by 16S rDNA-directed polymerase chain reaction. J Endod. 2001. 27:164–167.

6. Shenker BJ, Listgarten MA, Taichman NS. Suppression of human lymphocyte responses by oral spirochetes : A monocyte-dependent phenomenon. J Immunol. 1984. 132:2039–2045.

7. Shenker BJ, Datar S. Fusobacterium nucleatum inhibits human T-cells by arresting cells in the mid G₁ phase of the cell cycle. Infect Immun. 1995. 63:4830–4836.

8. Schumid I, Uittenbogaart CH, Giorgi JV. A gentle fixation and permeablization method for combined cell surface and intracellular staining with improved precision in DNA quantification. Cytometry. 1991. 12:279–285.

9. Shenker BJ, Mcarthur WP, Tsai CC. Immune suppression induced by Actinobacillus actinomycetemcomitans. I. Effects on human peripheral blood lymphocyte responses to mitogens and antigens. J Immunol. 1982. 128:148–154.

10. Yoshida H, Sundqvist G, Bergenholtz G. Effect of sonicated material from Fusobacterium nucleatum on the functional capacity od accessory cells derived from dental pulp. Oral Microbiol Immunol. 1995. 10:208–212.

11. Holmgren J, Lindholm L, Lonnroth I. Interaction of cholera toxin and toxin derivatives with lymphocytes. I. binding properties and interference with lectin-induced cellular stimulation. J Exp Med. 1974. 139:801–819.

12. Malakian AH, Schwab JH. Immunosuppressant from group A streptococci. Science. 1968. 159:880–881.

13. Hanna EE, Watson DW. Host-parasite relationships among group A streptococci. IV. Suppression of antibody response by streptococcal pyrogenic exotoxin. J Bacteriol. 1968. 90:14–21.

14. Floersheim GL, Hopff WH, Gasser M, Bucher K. Impairment of cell mediated immune response by Pseudomonas aeruginosa. Clin Exp Immunol. 1971. 9:241–247.

15. Khansari N, Segre M, Segre D. Immunosuppression in murine malaria : a soluble immunosuppressive factor derived from Plasmodium berghei-infected blood. J Immunol. 1981. 127:1889–1893.

16. Kauffman CA, Phair JP, Linneman CC, Schiff GM. Cell-mediated immunity in humans during viral infection. I. Effect of rubella on dermal hypersensitivity, PHA response and T lymphocytes numbers. Infect Immun. 1974. 10:212–215.

17. Olson GB, Dent PB, Rawls WE. Abnormalities of in vitro lymphocyte responses during rubella virus infections. J Exp Med. 1968. 128:47–68.

18. Kantzler GB, Lauteria SF, Cusumano CL, Lee JD, Ganguly R, Waldman RH. Immunosuppression during influenza virus infection. Infect Immun. 1974. 10:996–1002.

19. Engers HD, Louis JA, Zubler RH, Hirt B. Inhibition of Tx cell-mediated functions by MVM(i), a parvovirus closely related to minute virus of mice. J Immunol. 1981. 127:2280–2285.

20. Cianciola LJ, Genco RJ, Peters MR, McKenna J, VanOss CJ. Defective polymorphonuclear leukocyte function in a human periodontal disease. Nature. 1977. 265:445–447.

21. Clark RA, Page RC, Wilde G. Defective neutrophil chemotaxis in juvenile periodontitis. Infect Immun. 1977. 18:694–700.

22. Van Dyke TE, Horoszewicz HH, Cianciola LJ, Genco RJ. Neutrophil chemotaxis dysfunction in human periodontitis. Infect Immun. 1980. 27:124–132.

23. Schwab JH. Suppression of the immune response by microorganisms. Bacteriol Rev. 1975. 39:121–143.

24. Shenker BJ, Tsai CC, Taichman NS. Suppression of lymphocyte responses by Actinobacillus actinomycetemcomitans. J Periodontal Res. 1982. 17:462.