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Detection of Anti-Extractable Nuclear Antigens in Patients with Systemic Rheumatic Disease via Fluorescence Enzyme Immunoassay and Its Clinical Utility

Oh J, Park Y, Lee KA, Kim HS

PURPOSE: Testing for autoantibodies to extractable nuclear antigens (ENAs) plays an important role in the diagnosis and management of systemic rheumatic disease. Currently, no gold standard tests are available for...
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Inhibitory Effects of Protopanaxadiol on Lipopolysaccharide-Induced Reactive Oxygen Species Production and MUC5AC Expression in Human Airway Epithelial Cells

Song YS, Kim JH, Na H, Choi YS, Song SY, Kim YD, Bae CH

BACKGROUND AND OBJECTIVES: MUC5AC is one of the major secretory mucin genes in the human airway epithelium. MUC5AC expression is increased by a variety of inflammatory mediators. Protopanaxadiol (PPD), one...
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The evaluation of cellular immune function in elderly patients with systemic lupus erythematosus

Men K, Chen Y, Zhang J, Wei D

BACKGROUND/AIMS: To evaluate cellular immune function in systemic lupus erythematosus (SLE) patients over 60 years old, the association between antinuclear antibody (ANA) titers and the ratio of CD4⁺/CD8⁺ was analyzed...
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Endoplasmic Reticulum Stress Induces MUC5AC and MUC5B Expression in Human Nasal Airway Epithelial Cells

Kim MH, Bae CH, Choi YS, Na HG, Song SY, Kim YD

OBJECTIVES: Endoplasmic reticulum (ER) stress is known to be associated with inflammatory airway diseases, and three major transmembrane receptors: double-stranded RNA-activated protein kinase-like ER kinase, inositol requiring enzyme 1, and...
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Laboratory Diagnosis of Clostridium difficile Infection in Korea: The First National Survey

Chung HS, Park JS, Shin BM

In May 2015, we conducted a voluntary online survey on laboratory diagnostic assays for Clostridium difficile infection (CDI) across clinical microbiology laboratories in Korea. Responses were obtained from 66 laboratories,...
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Neopterin Levels and Indoleamine 2,3-Dioxygenase Activity as Biomarkers of Immune System Activation and Childhood Allergic Diseases

Ünüvar S, Erge D, Kılıçarslan B, Gözükara Bağ HG, Çatal F, Girgin G, Baydar T

BACKGROUND: Although Th2 immune activation is predominant in allergic diseases, neopterinlevels and indoleamine 2,3-dioxygenase (IDO)-1 activity (kynurenine:tryptophan ratio), which reflect Th1 immune activity, increase with interferon-gamma (IFN-γ) stimulation. We investigated...
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Nkx-2.5 Regulates MDR1 Expression via Its Upstream Promoter in Breast Cancer Cells

Lim JS, Jung GY, Park SY

BACKGROUND: Increased expression of MDR1 gene is one of the major mechanisms responsible for multidrug resistance in cancer cells. Two alternative promoters, upstream and downstream, are responsible for transcription of...
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Performance Evaluation of Two Immunochromatographic Assays for Rotavirus Detection in Stool Specimens

Kim S, Kim YJ, Cho SY, Park TS, Lee HJ

BACKGROUND: Rotavirus is a major pathogen causing enteritis worldwide in children under five years of age. In recent years, immunochromatographic assay (ICA) has been widely used as a diagnostic test...
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Performance of Hepatitis B Core-Related Antigen Versus Hepatitis B Surface Antigen and Hepatitis B Virus DNA in Predicting HBeAg-positive and HBeAg-negative Chronic Hepatitis

Zhang ZQ, Wang YB, Lu W, Liu DP, Shi BS, Zhang XN, Huang D, Li XF, Zhou XL, Ding RR

BACKGROUND: We examined changes in hepatitis B core-related antigen (HBcrAg) during the four sequential phases of chronic hepatitis B virus (HBV) infection: hepatitis B e antigen (HBeAg)-positive chronic infection (EPCI)...
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Triptolide Inhibits Lipopolysaccharide-Induced MUC5AC/5B Expression via Nuclear Factor-Kappa B in Human Airway Epithelial Cells

Seo BH, Choi TY, Choi YS, Bae CH, Na HG, Song SY, Kim YD

BACKGROUND AND OBJECTIVES: The representative mucin genes in the human airway are MUC5AC and MUC5B, which are regulated by several inflammatory and anti-inflammatory substances. Triptolide (TPL), udenafil, betulinic acid, changkil...
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Implementing Expanded Rapid Human Immunodeficiency Virus Testing in Public Health Centers in Seoul, 2015

Kang CR, Bang JH, Cho SI, Lee YH, Oh MD, Lee JK

In 2015, rapid human immunodeficiency virus (HIV) testing was implemented in all 25 public health centers in Seoul. During March and December 2015, 20,987 rapid HIV tests were performed, of...
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Usefulness of fecal calprotectin by monoclonal antibody testing in adult Japanese with inflammatory bowel diseases: a prospective multicenter study

Nakamura S, Imaeda H, Nishikawa , Iimuro M, Matsuura M, Oka H, Oku J, Miyazaki T, Honda H, Watanabe K, Nakase H, Andoh A

BACKGROUND/AIMS: Noninvasive objective monitoring is advantageous for optimizing treatment strategies in patients inflammatory bowel disease (IBD). Fecal calprotectin (FCP) is superior to traditional biomarkers in terms of assessing the activity...
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Decreased Serum Glutamate Levels in Male Adults with Internet Gaming Disorder: A Pilot Study

Paik SH, Choi MR, Kwak SM, Bang SH, Kim DJ

OBJECTIVE: Alteration in glutamatergic neurotransmission and dopaminergic dysfunction has been implicated in both the initiation and expression of addiction related behaviors. This pilot study was aimed to investigate the serum...
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Clusterin Induces MUC5AC Expression via Activation of NF-κB in Human Airway Epithelial Cells

Bae CH, Na HG, Choi YS, Song SY, Kim YD

OBJECTIVES: Clusterin (CLU) is known as apolipoprotein J, and has three isoforms with different biological functions. CLU is associated with various diseases such as Alzheimer disease, atherosclerosis, and some malignancies....
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Is Clostridium difficile infection a real threat in patients with ulcerative colitis? A prospective, multicenter study in Korea

Kim DB, Lee KM, Park SH, Kim YS, Kim ES, Lee J, Jung SA, Seo GS, Lee JM

BACKGROUND/AIMS: Clostridium difficile infection (CDI) has been reported to be a cause of flare-ups in patients with ulcerative colitis (UC). We evaluated the prevalence and clinical outcomes of CDI in...
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High Concentration of Glucose Induces MUC5B Expression via Reactive Oxygen Species in Human Airway Epithelial Cells

Lee YH, Kim YD, Song YS, Bae CH, Choi YS, Shin KC, Jin HJ, Song SY

BACKGROUND AND OBJECTIVES: The effects of hyperglycemia on the mucin secretion in inflammatory respiratory diseases are not clear. Therefore, this study was conducted to characterize the effect of hyperglycemia, and...
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Asian Sand Dust Up-Regulates MUC4 Expression in Human Upper Airway Epithelial Cells

Park CH, Song YS, Bae CH, Choi YS, Song SY, Shin KC, Jin HJ, Kim YD

BACKGROUND AND OBJECTIVES: Asian sand dust (ASD) is a meteorological phenomenon that occurs in spring time in Korea. ASD is composed of various organic and inorganic materials, which induce airway...
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A Simple Outline of Methods for Protein Isolation and Purification

Lee CH

At the summer workshop of the Korean Endocrine Society held in 2016, some examples of protein experiments were discussed in the session entitled “All about working with proteins.” In contrast...
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Effect of High Glucose on MUC5B Expression in Human Airway Epithelial Cells

Ye SB, Choi YS, Choi YH, Bae CH, Kim YW, Park SY, Song SY, Kim YD

OBJECTIVES: Excessive production of mucus results in plugging of the airway tract, which can increase morbidity and mortality in affected patients. In patients with diabetes, inflammatory airway disease appears with...
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Next-generation sequencing enables the discovery of more diverse positive clones from a phage-displayed antibody library

Yang W, Yoon A, Lee S, Kim S, Han J, Chung J

Phage display technology provides a powerful tool to screen a library for a binding molecule via an enrichment process. It has been adopted as a critical technology in the development...
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