J Korean Assoc Oral Maxillofac Surg.  2004 Aug;30(4):308-315.

The inductive capacity of primary cultured oral mucosal keratinocytes in skin wound healing of athymic nude mice

Affiliations
  • 1Department of Oral Pathology, Oral Cancer Research Institute, Yonsei University College of Dentistry, Brain Korea 21 project for Medical Science.
  • 2Department of Oral and Maxillofacial Surgery, Oral Cancer Research Institute, Yonsei University College of Dentistry, Brain Korea 21 project for Medical Science.

Abstract

AIM: The aim of this study was to investigate the mechanism of promoted skin wound healing in skin defects with primary cultured oral mucosal keratinocytes.
MATERIALS AND METHODS
Thirty adult female nude mice weighing 20+/-2g were used for the experiment. Primary cultured and suspended oral mucosal keratinocytes, labeled with BrdU, were scattered onto 1.5cm*1.5cmsized full thickness skin defects in the experimental group(N=15), and no grafts were placed the control group(N=15). They were sacrificed at 3 days, 1 week and 2 weeks after the treatment respectively. Histological examination of each wounds were performed to review the healing progress on measuring the length from the wound margin to regenerating epithelial front. The role of keratinocytes were assessed by double immunohistochemical staining with Anti-BrdU and Anti-cytokeratin AE1/3.
RESULTS
In the experimental group the wound was completely covered with regenerating epithelia in 2 weeks, but partially regenerated in the control group. The immunohistochemical studies unexpectedly reveal that most of regenerating epithelial cells were induced from marginal epithelium of the margin, not from the scattered keratinocytes.
CONCLUSION
We could successfully confirm that graft of primary cultured oral mucosal keratinocytes promotes the regeneration of skin defects.

Keyword

Oral mucosal keratinocyte; Primary culture; Wound healing; Tissue engineering

MeSH Terms

Adult
Animals
Bromodeoxyuridine
Epithelial Cells
Epithelium
Female
Humans
Keratinocytes*
Mice
Mice, Nude*
Regeneration
Skin*
Tissue Engineering
Transplants
Wound Healing*
Wounds and Injuries*
Bromodeoxyuridine
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