Korean J Thorac Cardiovasc Surg.
2007 Jul;40(7):467-472.
Changes of Serum IgM, IgG in Pig's Xenograft Perfusion and Immunofluorescence Changes of the Deposition of IgM, IgG in the Xenograft in Dogs
- Affiliations
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- 1Department of Thoracic and Cardiovascular Surgery, Yongdong Severance Hospital, Institute of Chest Disease, Yonsei University College of Medicine, Korea. dylee@yumc.yonsei.ac.kr
- 2Department of Diagnostic Pathology, Yongdong Severance Hospital, Yonsei University College of Medicine, Korea.
- 3Department of Thoracic and Cardiovascular Surgery, Eulji University Hospital, Eulji University, Korea.
- 4Department of Thoracic and Cardiovascular Surgery, Graduate School of Medicine, Kyoto University Kyoto, Japan.
Abstract
- BACKGROUND
Lung transplantation is the definitive therapy for end stage lung disorders. The success of allogenic lung transplantation has led to an increasing shortage of donor lungs from humans, including cadavers, and attention has now turned to transplantation of lungs from other species. However, there are many biological hurdles when using organs from other species because of hyperacute rejection after discordant xenotransplantation. MATERIAL AND METHOD: Pigs (n=6, weighing 20~30 kg each) for the donors and mongrel dogs (n=6, weighing 20~28 kg each) for the recipients were used in this experiment. The left kidney of a pig was perfused to a mongrel dog for 30 minutes through the femoral artery and vein of the dog, and the right kidney was perfused for 30 minutes sequentially. Then, both lungs of the pig were perfused to the dog through the pulmonary artery and left atrium with using the same time intervals. The levels of IgM and IgG were measured from the blood and specimens of the kidney and lung. RESULT:The average levels of serum IgM gradually decreased after the perfusion, but the average levels of serum IgG did not change from before to after perfusion. The immunohistochemical findings revealed decreased deposition of IgG and IgM after the perfusion. CONCLUSION: We conclude that the levels of the serum natural antibodies would be decreased with pre-transplantation xenograft perfusion in the recipient and the occurrence rate of hyperacute rejection after transplantation would be decreased.
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