Korean J Anesthesiol.
2006 Feb;50(2):188-197. 10.4097/kjae.2006.50.2.188.
The Protective Effect of Ischemic and Hypoxic Preconditioning on Hypoxic-ischemic Brain Injury in the Neonatal Rat: 1H Magnetic Resonance Spectroscopic Study
- Affiliations
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- 1Department of Anesthesiology and Pain Medicine, College of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea. phpark@amc.seoul.kr
- 2Department of Anesthesiology and Pain Medicine, Kangneung Asan Medical Center, Gangneung, Korea.
- 3NMR Laboratory, Asan Institute for Life Sciences, Seoul, Korea.
- KMID: 2237458
- DOI: http://doi.org/10.4097/kjae.2006.50.2.188
Abstract
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BACKGROUND: A brief episode of cerebral ischemia confers transient ischemic tolerance to a subsequent ischemic challenge. We examined the effect of ischemic and hypoxic preconditioning in the neonatal rat.
METHODS
Seven-day old Sprague-Dawley rat pups were divided into three groups:control (n = 53), ischemic preconditioning (n = 51), and hypoxic preconditioning (n = 48). For ischemic preconditioning, the right common carotid artery was occluded for 10 min. Rats in the hypoxic preconditioning group were kept under hypoxic (8% oxygen/92% nitrogen) conditions for 4h. Twenty-four hours after the preconditioning, rats from all groups were exposed to the right common carotid artery ligature, followed by 2.5 h of hypoxia. Lipid/N-acetyl aspartate (Lip/NAA) and lipid/creatine (Lip/Cr) ratios from 1H MR spectroscopy and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) were evaluated as measures of apoptosis 1 and 7 days after hypoxic-ischemic injury.
RESULTS
In the ischemic and hypoxic preconditioning groups, the Lip/NAA and Lip/Cr ratios and the numbers of TUNEL-positive cells were significantly lower than those in the control group (P < 0.05), but there were no significant differences between the two preconditioning groups.
CONCLUSIONS
These results suggest that ischemic and hypoxic preconditioning in the neonatal rat attenuate the apoptosis that is caused by hypoxic-ischemic brain injury.
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