Korean J Anesthesiol.  2006 Jun;50(6):698-705. 10.4097/kjae.2006.50.6.698.

Vascular Response of Isolated Rat Thoracic Aorta after Chemical Hypoxia/Reoxygenation

• Jang BS
• Kim IK
• Lim DG

• KMID: 1862248
• DOI: http://doi.org/10.4097/kjae.2006.50.6.698

Abstract

BACKGROUND: Hypoxia/reoxygenation (H/R) results in formation of toxic reactive oxygen species (ROS), which can impair the vascular pathophysiology. Nitric oxide (NO) is an important free radical in many physiological or pathological processes including H/R injury. The loss of NO after H/R might be one of the major causes of an impaired vascular response.
METHODS
Isolated rat aortic rings were prepared and NaCN was used to induce chemical hypoxia. The NaCN concentration and the hypoxia/reoxygenation time were determined by the responsiveness of phenylephrine (Phe), sodium nitroprusside (SNP) and acetylcholine (Ach). A cumulative doses of Phe and SNP (10(-9)-10(-5.5) M) were added to construct the vascular contraction and relaxation curves. The cumulative doses of Ach (10(-9)-10(-5) M) were added to construct the relaxation after precontraction with Phe (10(-6) M). The effects of the N(G)-nitro-L-arginine methyl ester (L-NAME, 10(-4) M) and the superoxide dismutase (SOD, 50 unit) pretreatment during chemical H/R were evaluated.
RESULTS
The NaCN concentration and H/R time were 1 mM, 30 minutes/5 minutes, respectively. Chemical hypoxia reduced the Phe-induced vascular contraction significantly. However chemical H/R increased the Phe-induced contraction significantly, and impaired the relaxation by SNP and Ach. A pretreatment with L-NAME increased the Phe-induced contraction and impaired the relaxation by SNP as well as Ach. The SOD pretreatment reduced the Phe-induced increased vascular contraction after NaCN-induced chemical H/R.
CONCLUSIONS
NO plays a key role in endothelial-dependent relaxation and the recovery of the augmented contractility by vasoconstrictors after chemically-induced H/R.

Keyword

chemical hypoxia; hypoxia/reoxygenation; nitric oxide; N(G)-nitro-L-arginine methyl ester; reactive oxygen species; superoxide dismutase

MeSH Terms

Acetylcholine
Animals
Anoxia
Aorta, Thoracic*
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitroprusside
Pathologic Processes
Phenylephrine
Rats*
Reactive Oxygen Species
Relaxation
Superoxide Dismutase
Vasoconstrictor Agents
Acetylcholine
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitroprusside
Phenylephrine
Reactive Oxygen Species
Superoxide Dismutase
Vasoconstrictor Agents