Yonsei Med J.  2013 Jan;54(1):177-182. 10.3349/ymj.2013.54.1.177.

Molecular and Epidemiological Characterization of Carbapenem-Resistant Acinetobacter baumannii in Non-Tertiary Korean Hospitals

• Park S
• Kim HS
• Lee KM
• Yoo JS
• Yoo JI
• Lee YS
• Chung GT

• KMID: 1776935
• DOI: http://doi.org/10.3349/ymj.2013.54.1.177

Abstract

PURPOSE
The increasing prevalence and global spread of carbapenem-resistant Acinetobacter baumannii (A. baumannii) has become a serious problem. The aim of this study was to investigate molecular and epidemiological characteristics of carbapenem-resistant A. baumannii isolates collected from Korean non-tertiary hospitals.
MATERIALS AND METHODS
Thirty six non-duplicated carbapenem-resistant A. baumannii isolates were collected from 17 non-tertiary hospitals in Korea between 2004 and 2006. Isolates were typed by multilocus sequence typing and repetitive-sequence-based PCR (rep-PCR). Detection of genes encoding OXA carbapenemase and their relationship with ISAba1 was performed by PCR.
RESULTS
Two clones were prevalent among 36 isolates: ST69 (17 isolates, 47.2%) and ST92 (19 isolates, 52.8%). Rep-PCR patterns were diverse and revealed that all isolates were clustered into eight band patterns. The ISAba1-activated blaOXA-23-like and ISAba1-activated blaOXA-51-like genes were prevalent among the carbapenem-resistant A. baumannii isolates.
CONCLUSION
The class D beta-lactamase genes of A. baumannii were distributed nationwide in non-tertiary Korean hospitals.

Keyword

Acinetobacter baumannii; Carbapenem-resistant; OXA carbapenemase

MeSH Terms

Acinetobacter Infections/epidemiology/*microbiology
Acinetobacter baumannii/classification/*genetics
Anti-Bacterial Agents/therapeutic use
Bacterial Typing Techniques
Carbapenems/*therapeutic use
DNA, Bacterial/analysis
*Drug Resistance, Bacterial
Hospitals
Humans
Microbial Sensitivity Tests
Molecular Epidemiology
Multilocus Sequence Typing
Polymerase Chain Reaction
Prevalence
Republic of Korea
beta-Lactamases/genetics
Anti-Bacterial Agents
Carbapenems
DNA, Bacterial
beta-Lactamases

Figure

• 

  Fig. 1 Rep-PCR banding patterns and STs of 36 carbapenem resistant A. baumannii.

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