J Bacteriol Virol.  2006 Mar;36(1):21-30. 10.4167/jbv.2006.36.1.21.

Determination of Genomospecies and Characterization of Antimicrobial Resistance of Multi-drug Resistant Acinetobacter spp. Isolates

• Lim YM
• Choi TS
• Kim J

• KMID: 1719021
• DOI: http://doi.org/10.4167/jbv.2006.36.1.21

Abstract

Acinetobacter species are non-fermentative Gram-negative coccobacilli and they have emerged as important nosocomial pathogens which are associated with the significant multidrug resistance in recent years. Carbapenem-resistant A. baumannii (CRAB) and pandrug-resistant A. baumannii (PDRAB) were reported in 1991 and 1998, respectively. Fiftyeight isolates of Acinetobacter species recovered from a university hospital between August 2004 and March 2005 were investigated for the existence of CRAB, PDRAB, extended-spectrum beta-lactamase (ESBL)-producing Acinetobacter and examined for their phenotypic and genotypic characteristics. Genomospecies of Acinetobacter species were determined by amplified rDNA restriction analysis (ARDRA) and antimicrobial susceptibility test was performed with 13 kinds of antimicrobial agents. Metallo-beta-lactamase (MBL) producers were screened by modified hodge test and confirmed by imipenem-EDTA disk synergy test. Detection of blaIMP-1, blaVIM-2, blaTEM, and blaPER-1 was performed by PCR. Genomic DNAs were analyzed by pulsed-field gel electrophoresis (PFGE). Among 58 isolates of Acinteobacter species, 40 isolates were identified as genospecies 2 (A. baumannii), 9 were 13TU, 5 were A. phenon 6/ct, and 4 were Acinetobacter genospecies 3 by ARDRA. Thirteen isolates were confirmed as MBL-producers and blaIMP-1 and blaVIM-2 were carried by 5 and 8 isolates of them, respectively. MBL-producers were mostly 13TU, A. phenon 6/ct 13TU, and Acinetobacter genospecies 3 and they were susceptible to ciprofloxacin and ampicillin-sulbactam. BlaPER-1 was carried by thirteen isolates and 12 isolates of them were PDRAB showing resistance to all antimicrobial agents tested, including ceftazidime, cefepime, aztreonam, ciprofloxacin, amikacin, gentamicin, ampicillin-sulbactam, and imipenem. In conclusion, most MBL-producers belonged to 13TU, A. phenon 6/ct 13TU, and Acinetobacter genospecies 3 which were susceptible to ciprofloxacin and ampicillin-sulbactam, whereas 12 of 13 PER-1-producers were PDRAB originated from the same clone.

Keyword

Acinetobacter spp.; Genomic typing; Metallo-beta-lactamase; PER-1; Pan-drug resistant Acinetobacter baumannii

MeSH Terms

Acinetobacter*
Amikacin
Anti-Infective Agents
Aztreonam
beta-Lactamases
Ceftazidime
Ciprofloxacin
Clone Cells
DNA
DNA, Ribosomal
Drug Resistance, Multiple
Electrophoresis, Gel, Pulsed-Field
Gentamicins
Imipenem
Polymerase Chain Reaction
Amikacin
Anti-Infective Agents
Aztreonam
Ceftazidime
Ciprofloxacin
DNA
DNA, Ribosomal
Gentamicins
Imipenem
beta-Lactamases

Figure

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  Figure 1. Restriction patterns obtained after restriction digestion with CfoI, AluI, MboI, RsaI, and MspI for amplified 16S rDNA of different Acinetobacter species. Numbers below each lane correspond to arbitrarily assigned ARDRA pattern numbers for each enzyme (http://users.ugent.be/∼mvaneech/ARDRA/Acinetobacter.html).

• 

  Figure 2. Dendrogram generated by Gel Compar II software showing the relatedness of Acinetobacter baumannii determined by PFGE.

Reference

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