Yonsei Med J.  2006 Feb;47(1):70-77. 10.3349/ymj.2006.47.1.70.

Expression of Ca2+-dependent Synaptotagmin Isoforms in Mouse and Rat Parotid Acinar Cells

Affiliations
  • 1Department of Oral Biology, Brain Korea 21 Project of Medical Science, Yonsei University College of Dentistry, Seoul, Korea.
  • 2Department of Pedodontics, Yonsei University College of Dentistry, Seoul, Korea. dmshin@yumc.yonsei.ac.kr

Abstract

Synaptotagmin is a Ca2+ sensing protein, which triggers a fusion of synaptic vesicles in neuronal transmission. Little is known regarding the expression of Ca2+ - dependent synaptotagmin isoforms and their contribution to the release of secretory vesicles in mouse and rat parotid acinar cells. We investigated a type of Ca2+ - dependent synaptotagmin and Ca2+ signaling in both rat and mouse parotid acinar cells using RT-PCR, microfluorometry, and amylase assay. Mouse parotid acinar cells exhibited much more sensitive amylase release in response to muscarinic stimulation than did rat parotid acinar cells. However, transient [Ca2+]i increases and Ca2+ influx in response to muscarinic stimulation in both cells were identical, suggesting that the expression or activity of the Ca2+ sensing proteins is different. Seven Ca2+ - dependent synaptotagmins, from 1 to 7, were expressed in the mouse parotid acinar cells. However, in the rat parotid acinar cells, only synaptotagmins 1, 3, 4 and 7 were expressed. These results indicate that the expression of Ca2+ - dependent synaptotagmins may contribute to the release of secretory vesicles in parotid acinar cells.

Keyword

Synaptotagmin; calcium signaling; exocytosis; parotid acinar cells

MeSH Terms

Synaptotagmins/*metabolism
Signal Transduction
Rats
Protein Isoforms/metabolism
Parotid Gland/cytology/*metabolism
Muscarinic Agonists/pharmacology
Mice
Exocytosis/drug effects/physiology
Carbachol/pharmacology
Calcium/metabolism/*physiology
Animals
Amylases/secretion
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