J Bacteriol Virol.
2003 Sep;33(3):177-181.
Detection of Leptospiral DNA from Field Rodents by PCR
- Affiliations
-
- 1Department of Microbiology, College of Medicine, Hallym University, Chunchon 200-702 Korea. mkcho@hallym.ac.kr
- 2Institute of Medical Science, College of Medicine, Hallym University, Chunchon 200-702 Korea.
- 3Department of Microbiology, College of Medicine, Korea University, Seoul 140-220 Korea.
- 4Institute for Viral Disease, College of Medicine, Korea University, Seoul 140-220 Korea.
- 5US Army 485th Medical Detachment PM, Fort Polk, LA 71459-7434 U.S.A.
- 6US Army 18th Medcom, Preventive Services Directorate, Seoul 140-220 Korea.
Abstract
- Leptospirosis has been one of important epidemic diseases in Korea since 1984. Wild rodents, mostly Apodemus agrarius, served the important source of infection especially in harvest season in rural areas of Korea. Prevalence of Leptospira spp. infection in field rodents were investigated by detecting leptospiral DNA from rodent kidney. Among 108 rodents collected from various areas in 1998, leptospiral DNA were detected from 7 rodents (6.48%). Among 104 rodents, Apodemus agrarius, captured from Yeonchon and Paju area in 2001 and 2002, leptospiral DNA were detected from 6 rodents (5.76%). No leptospiral DNA was detected from 23 rodonts (Apodemus peninsulae 16, Apodemus agrarius 2 and Eothenomys regulus 5) captured in Odae mountain area in 1998.