Korean J Aerosp Environ Med.  2005 Dec;15(3):93-99.

Relationship between Mitochondrial DNA Point Mutation and Noise-induced Sensorineural Hearing Loss

Affiliations
  • 1Department of Preventive Medicine, Keimyung University School of Medicine, Daegu, Korea. dhshin@dsmc.or.kr
  • 2Department of Physiology, Keimyung University School of Medicine, Daegu, Korea.
  • 3Department of Occupational and Environmental Medicine, Keimyung University Dongsan Medical Center and Institute for Medical Genetics, Keimyung Universityity, Daegu, Korea.

Abstract

BACKGROUND
A different sequence change in the mitochondrial 12S rRNA gene has been proposed as a candidate mutation in the sensorineural hearing loss. The purpose of this study was to study the association between the noise-induced sensorineural hearing loss and A to G mutation at nucleotide 1555 of mitochondrial DNA.
METHODS
subjects were reviewed by history and medical records, and audiological and clinical data were obtained. Blood sampling was done on 101 normal controls, 51 with noise-induced sensorineural hearing loss, and 12 with sensorineurnal deafness. The DNA of these individuals were extracted, and mitochondrial genome were analyzed by polymerase chain reaction (PCR). Subsequently, the coding sequence of mitochondrial genome was sequenced and compared to the normal sequence, and all sequence variations were analyzed by restriction endonuclease BsmA I.
RESULTS
Mitochondrial DNA mutant (1555A-->G) was not detected by PCR in all Korean patients with noise-induced hearing loss, sensorineural hearing loss, and in normal controls with no hearing loss. The DNA sequencing of PCR products did not reveal an A to G substitution at nucleotide 1,555 of mitochondrial DNA.
CONCLUSION
This result suggests that the noise-induced sensorineural hearing loss is not associated with mitochondrial DNA mutation (1555A-->G).

Keyword

Mitochondrial DNA mutation; Noise-induced sensorineural hearing loss

MeSH Terms

Clinical Coding
Deafness
DNA
DNA Restriction Enzymes
DNA, Mitochondrial*
Genes, rRNA
Genome, Mitochondrial
Hearing Loss
Hearing Loss, Noise-Induced
Hearing Loss, Sensorineural*
Humans
Medical Records
Point Mutation*
Polymerase Chain Reaction
Sequence Analysis, DNA
DNA
DNA Restriction Enzymes
DNA, Mitochondrial
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