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Cloning, expression and purification flagellar sheath adhesion of Helicobacter pylori in Escherichia coli host as a vaccination target

Soleimani , Mohabati Mobarez A, Farhangi B

PURPOSE: Helicobacter pylori is a widely distributed gram-negative bacterium that infects the human stomach and duodenum. HpaA is a H. pylori-specific lipoprotein that has been shown to be an effective...
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In Silico Structural and Functional Annotation of Hypothetical Proteins of Vibrio cholerae O139

Islam MS, Shahik SM, Sohel M, Patwary NI, Hasan MA

In developing countries threat of cholera is a significant health concern whenever water purification and sewage disposal systems are inadequate. Vibrio cholerae is one of the responsible bacteria involved in...
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Screening of Epidermal Growth Factor Receptor Gene Mutation in Non-Small Cell Lung Cancer Using a PCR-Based Enzymatic Digestion Method

Jung YJ, Park SJ, Lee SB, Kim YT, Park JY, Park IK, Kang CH, Kim JH

PURPOSE: We applied a simplified method using polymerase chain reaction (PCR)-based enzymatic digestion for the detection of epidermal growth factor receptor (EGFR) mutation. MATERIALS AND METHODS: We selected 74 samples of...
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Association of Estrogen Receptor 2(ESR 2) Gene Polymorphisms with Ossification of the Posterior Longitudinal Ligament of the Spine

Kim KT, Lee SH, Kwack YH, Son ES, Park KJ, Kim DH

STUDY DESIGN: Genetic screening of the estrogen receptor 2 (ESR2) genes in patients with ossification of the posterior longitudinal ligament (OPLL). OBJECTIVE: We studied the relationships between ESR2 gene polymorphisms and...
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Vitamin D Receptor Gene TaqI, BsmI and FokI Polymorphisms in Korean Patients with Tuberculosis

Kang TJ, Jin SH, Yeum CE, Lee SB, Kim CH, Lee SH, Kim KH, Shin ES, Chae GT

BACKGROUND: The active metabolite (1, 25-dihydroxycholecalciferol) of vitamin D (25-hydroxycholecalciferol) leads to activation of macrophages and deficiency of vitamin D seems to be involved in the risk of tuberculosis. The...
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Multiplex PCR Detection of Waterborne Intestinal Protozoa; Microsporidia, Cyclospora, and Cryptosporidium

Lee SH, Joung M, Yoon S, Choi K, Park WY, Yu JR

Recently, emerging waterborne protozoa, such as microsporidia, Cyclospora, and Cryptosporidium, have become a challenge to human health worldwide. Rapid, simple, and economical detection methods for these major waterborne protozoa in...
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Determination of Porcine Rotavirus Serotypes by RT-PCR and RFLP Analysis

Min HG, Lim YH, Kang SY

G and P tying of group A porcine rotaviruses (P(o)RV) from field fecal samples were performed using reversetranscriptase polymerization chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP) analysis. After...
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Effect of nerve growth factor gene injection on the nerve regeneration in rat lingual nerve crush-injury model

Gao EF, Chung HJ, Ahn KM, Kim SM, Kim YH, Jahng JW, Lee JH

  • KMID: 2058616
  • J Korean Assoc Maxillofac Plast Reconstr Surg.
  • 2006 Sep;28(5):375-395.
PURPOSE: Lingual nerve (LN) damage may be caused by either tumor resection or injury such as wisdom tooth extraction, Although autologous nerve graft is sometimes used to repair the damaged...
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A Case Report of Familial Benign Hypocalciuric Hypercalcemia: A Mutation in the Calcium-Sensing Receptor Gene

Woo SI, Song H, Song KE, Kim DJ, Lee KW, Kim SJ, Chung YS

Familial benign hypocalciuric hypercalcemia (FBHH) is an autosomal dominant trait with high penetrance, clinically manifestating a relatively benign, lifelong, persistent hypercalcemia and hypocalciuria without hypercalcemic related complications. The calcium-sensing receptor...
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Relationship between Mitochondrial DNA Point Mutation and Noise-induced Sensorineural Hearing Loss

Park SH, Kim TW, Lee MY, Bae JH, Shin DH

  • KMID: 1589163
  • Korean J Aerosp Environ Med.
  • 2005 Dec;15(3):93-99.
BACKGROUND: A different sequence change in the mitochondrial 12S rRNA gene has been proposed as a candidate mutation in the sensorineural hearing loss. The purpose of this study was to...
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Single Nucleotide Polymorphisms in the 3'-Untranslated Region of Vascular Endothelial Growth Factor in Diabetic Retinopathy

Byeon SH, Oh HS, Ahn BY, Hong SP, Kim JK, Kwon OW

  • KMID: 2337231
  • J Korean Ophthalmol Soc.
  • 2005 Nov;46(11):1911-1918.
PURPOSE: Recent studies suggest that increased expression of the vascular endothelial growth factor (VEGF) may play a role in the pathogenesis of diabetic complications. The aim of this study was...
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Epidemiologic analysis of Ampicillin Resistance in Shigella sonnei Isolates by blaTEM Hybridization

Seol SY, Bae HK, Woo SY, Jeong YS, Yu HS, Lee YC, Cho DT

  • KMID: 2054983
  • J Bacteriol Virol.
  • 2004 Mar;34(1):19-26.
Forty-seven ampicillin-resistant R plasmids derived from 218 Shigella sonnei isolates from Daegu and Gwangju areas from 1980 to 2000 were epidemiologically compared by fragments of restriction endonuclease patterns by EcoRI...
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Genome Mapping of an Extreme Thermophile, Thermus caldophilus GK24

Park JH, Park BC, Koch SH, Kim JS, Koh JH, Yang MH, Kim YS, Kim CH, Kim MH, Kwon ST, Lee DS

  • KMID: 1973995
  • Genomics Inform.
  • 2003 Sep;1(1):50-54.
Genome of an extreme thermophile, Thermus caldophilus GK24 has been analyzed to construct the genomic map. The genomic DNAs encapsulated in agarose gel were digested with SspI, EcoRI, SpeI, and...
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Virulence Factors and Genotyping of Shigella sonnei Isolated from Patients

Kim YB, Moon JY, Chang CL

  • KMID: 2239629
  • Korean J Lab Med.
  • 2002 Dec;22(6):395-402.
BACKGROUND: Nineteen strains of Shigella sonnei isolated from the patients were examined regarding their biochemical characterization, serotype, and antibiotics resistance, and then analyzed for plasmid DNA profile. METHODS: Strains were tested...
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Epidemiologic analysis of Shigella sonnei Isolates by using Antimicrobial Resistance Gene Probes

Seol SY, Jeong YS, Kang HY, Yu HS, Lee YC, Cho DT

  • KMID: 2054957
  • J Bacteriol Virol.
  • 2002 Dec;32(4):347-354.
Thirty-four Shigella sonnei isolates from 6 outbreaks and sporadic cases from May 1999 until January 2000 in Daegu and 9 regions of Gyeongsangbuk-Do were epidemiologically analyzed by plasmid profiling, pulsed-field...
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Molecular Typing of Candida parapsilosis Isolates from Patients and Healthcare Workers by Pulsed-Field Gel Electrophoresis

Shin JH, Shin DH, Song JW, Park MR, Kim KJ, Cho D, Kee SJ, Suh SP, Ryang DW

  • KMID: 2248670
  • Korean J Infect Dis.
  • 2002 Oct;34(5):311-317.
BACKGROUND: The epidemiology of Candida parapsilosis is still undefined and may involve sources such as hospital environment and hands of healthcare workers (HCWs). We performed molecular typing of C. parapsilosis...
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Molecular Typing of Urinary Isolates of Candida tropicalis by Pulsed-Field Gel Electrophoresis (PFGE) and Random Amplified Polymorphic DNA (RAPD) Analysis

Rho J, Kim CS, Jang SJ, Shin JH, Suh SP, Ryang DW

  • KMID: 2038534
  • Korean J Infect Dis.
  • 2001 Dec;33(6):392-403.
BACKGROUND: Despite the recognized increase of frequency of candiduria due to Candida tropicalis, little was known of its molecular epidemiology. We applied PFGE and RAPD assay for urinary C. tropicalis...
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A Case of Knee Joint Infection by Mycobacterium abscessus

Ryu SW, Lee CK, Heo J, Shin KS, Kim JS, Bae SY, Chang EA, Suh IB, Kim YK, Lee KN

  • KMID: 2146108
  • Korean J Clin Pathol.
  • 2001 Oct;21(5):371-376.
Rapid growing mycobacterium grows in less than 7 days on most types of solid media including the Ogawa media. Ninety percent of human diseases caused by rapid growing mycobacterium are...
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Characterization of Type 2 Restriction Endonucleases (Hpy51) from Helicobacter pylori Strain 51

Cho MJ, Park JU, Jeon BS, Pack JW, Byun EY, Lee SK, Park YH, Song JY, Lee WK, Baik SC, Choi YJ, Jung SA, Choe MY, Choi SH, Ko GH, Youn HS, Rhee KH

  • KMID: 1571503
  • J Bacteriol Virol.
  • 2001 Sep;31(3):207-215.
This study describes the purification and characterization of type II restriction endonuclease of Helicobacter pylori in order to understand the DNA restriction and modification of H. pylori. H. pylori cell...
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Homozygous VN (677C to T) and d/D (2756G to A) variants in the methylenetetrahydrofolate and methionine synthase genes in a case of hyperhomocysteinemia with stroke at young age

Song KS, Song JW, Choi JR, Kim HK, Shin JS, Kim JH

  • KMID: 1085748
  • Exp Mol Med.
  • 2001 Jun;33(2):106-109.
Hyperhomocysteinemia is known to be associated with an increased risk of myocardial infarction, stroke, peripheral arterial disease, and venous thrombosis. Gene polymorphisms in methylenetetrahydrofolate reductase (MTHFR) and methionine synthase (MS)...
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