J Korean Rheum Assoc.
2006 Jun;13(2):140-149.
The Expression of Toll-like Receptors in Collagen-induced Arthritis
- Affiliations
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- 1Department of Internal Medicine, College of Medicine, Pusan National University, Busan, Korea. ksimd@pusan.ac.kr
- 2Research Institute, College of Medicine, Pusan National University, Busan, Korea.
Abstract
OBJECTIVE
To evaluate the expression of Toll-like receptor (TLR) 2, 4 and 9 and investigate the effects of IL-17 on the expression of TLRs in experimental rheumatoid arthritis (RA) model.
METHODS
After induction of collagen-induced arthritis (CIA) by type II collagen in DBA1 mice, phosphate-buffered saline (PBS, PBS group) or IL-17 (IL-17 group) was injected to both knee joint at day 28 and 32. At day 35, mice were sacrificed and knee joints were isolated. Synovial mRNA expressions of TLR-2, 4 and 9 determined by real-time RT-PCR were compared among normal DBA1 mice (normal group), PBS and IL-17 group.
RESULTS
Synovial TLR-2, 4, and 9 mRNA expressions of IL-17 and PBS group were significantly higher than normal group, and those of IL-17 group were higher than PBS group.
CONCLUSION
Synovial TLR-2, 4 and 9 expression was enhanced in CIA and up-regulated by local overexpression of IL-17. These results suggest that TLRs play a roles on CIA and IL-17 induced aggravation of arthritis in CIA.
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