J Korean Soc Virol.  1999 Sep;29(3):155-163.

Molecular Characterization of the L Segment of Hantann Virus, Strain Howang

• Chu YK
• Song DY
• Koo HM
• Lee HW

Abstract

Hantaan virus (HTNV), the etiologic agent of hemorrhagic fever with renal syndrome (HFRS), belongs to the genus Hantavirus, and has three single negative straded RNA genome segments. HTNV strain Howang isolated from the blood of severe case of Korean HFRS is more virulent than HTNV 76/118 and the M and S genome segments' nucleotide sequence of Howang strain showed 93.5% and 94% homology to each segment of HTNV 76/118. We have obtained 6533 nucleotides long sequence of the L genome segment of Howang strain using reverse transcriptase in conjunction with PCR amplification and compared to other hantaviruses. The messenger sense of the L segment contains one long single long open reading frame of 2151 amino acids, which encodes a deduced RNA dependent RNA polymerase of 246.4 kDa caculated molecular weight protein. The nucleotide sequence of the Lsegment of Howang strain shows 93%, 74%, 66%,65% homology to HTNV 76/118, Seoul virus 80/39, Puumala virus Hallnas B1 and Sin Nombre virus, respectively. The amino acid sequence of the L segment of Howang strain shows 99%, 85%, 68%, 68% homology to HTNV 76/118, Seoul virus 80/39, Puumala virus Hallnas B1 and Sin Nombre virus, respectively.

Keyword

Hantavirus; Hantaan virus strain Howang; L segment; Nucleotide sequence

MeSH Terms

Amino Acid Sequence
Amino Acids
Base Sequence
Genome
Hantaan virus
Hantavirus
Hemorrhagic Fever with Renal Syndrome
Molecular Weight
Nucleotides
Open Reading Frames
Polymerase Chain Reaction
Puumala virus
RNA
RNA Replicase
RNA-Directed DNA Polymerase
Seoul virus
Sin Nombre virus
Amino Acids
Nucleotides
RNA
RNA Replicase
RNA-Directed DNA Polymerase