Korean J Anat.
1999 Jun;32(3):279-292.
Immunohistochemical Studies on Striatal Dopamine Recovery by Cell Transplantation in the Parkinsonian Rat Model
- Affiliations
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- 1Department of Anatomy, Seoul National University College of Medicine, Seoul 110-799, Korea.
- 2Department of Neurosurgery, Seoul National University College of Medicine, Seoul 110-799, Korea.
- 3Department of Pharmacology, Seoul National University College of Medicine, Seoul 110-799, Korea.
- 4Department of MRC Neuroscience Research Institute, Seoul National University College of Medicine, Seoul 110-799, Korea.
Abstract
- We have used a rat model of Parkinson's disease (PD) to address issues of importance for a future clinical application of dopamine neuron grafting in patients with PD. Implantation of fetal dopamine containing tissue into the striatum of Parkinson's disease patients or animal PD model leads to behaviral recovery. Recovery may be related to two sources of dopamine: the grafted cells and/or the sprouted fibers from host dopaminergic neurons. In this study, we attended to determine whether behavoral recovery requires release of dopamine by the transplanted tissue or the sprouted fibers. Hemiparkinsonian model was made by 6-hydroxydopamine (6-OHDA) induced destruction of the dopaminergic migrostrial pathway only (selective model), or including mesolimbic pathway (complete model) which were confirmed by histochemical analysis. Either embryonic mesencephalic neurons or tyrosine hydroxylase (TH) gene-transfected astrocytes were microtransplanted into multiple striatal sites. Rats showing behavoral recovery by transplantation were sacrified and analysed by TH immunohistochemical staining. Strong TH-positive cell bodies and fibers extending from them appeared in the rats with embryonic mesencephalic neuron transplantation. However only TH-positive cell bodies were observed in TH-gene-transfected astrocyte transplants. No obvious sprouting of dopaminergic fibers from host striatal tissue were observed in both selective and complete rat PD models. It is concluded that only dopaminergic cell implants are able to induce functional recovery in 6-OHDA induced parkinsonian rats.
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