Immune Netw.  2004 Dec;4(4):250-254. 10.4110/in.2004.4.4.250.

Enhancement of the Stability and Skin Penetration of Vitamin C by Polyphenol

Affiliations
  • 1Department of Anatomy and Tumor Immunity, Medical Reaserch Center, Seoul National University College of Medicine, Korea. Kinglee@snu.ac.kr
  • 2Department of Life Science, Sookmyung Women's University, Korea.

Abstract

BACKGROUND
It is necessary for human beings to uptake vitamin C through diet or supplements. It is also well-known that vitamin C plays an important role in the prevention of scurvy, enhancement of collagen synthesis and anti-tumor immune response. In addition, there are several recent reports regarding the effective role of vitamin C on the regulation of allergic responses, such as atopic dermatitis and asthma. However, the effective therapeutic and preventive measures using vitamin C are not established yet, since vitamin C is seriously unstable in aqueous solution. Therefore, we have investigated the best way to maintain the stability of vitamin C. METHODS: After we making a mixture of polyphenol (0.001, 0.01, 0.1%) and vitamin C (1 mM), the mixtures were placed at room temperature both with/without light protection. And then the concentration of ascorbic acid was measured with HPLC. To analyze the in vivo effect of vitamin C on the regulation of skin allergic reaction, polyphenol (0.1%)-vitamin C (1 mM) mixture was applied to the skin and the production of histamine from mast cell was analyzed by Evans blue dye staining. RESULTS: We have found that the polyphenol has preventive power of oxidation of vitamin C. In addition, the production of histamine was suppressed by the polyphenol (0.1%)-vitamin C (1 mM) mixture. CONCLUSION: We have reached the conclusion that our study suggests the research guideline for the therapy of atopic dermatitis through vitamin C.

Keyword

Vitamin C; polyphenol; histamine; atopic dermatitis

MeSH Terms

Ascorbic Acid*
Asthma
Chromatography, High Pressure Liquid
Collagen
Dermatitis, Atopic
Diet
Evans Blue
Histamine
Humans
Hypersensitivity
Mast Cells
Scurvy
Skin*
Vitamins*
Ascorbic Acid
Collagen
Evans Blue
Histamine
Vitamins
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