Immune Netw.  2009 Aug;9(4):133-137. 10.4110/in.2009.9.4.133.

Further Characterization of Activin A-induced IgA Response in Murine B Lymphocytes

• Lee HJ
• Kim PH

• KMID: 1474585
• DOI: http://doi.org/10.4110/in.2009.9.4.133

Abstract

We have recently shown that activin A, a member of TGF-beta superfamily, stimulates mouse B cells to express IgA isotype but other isotypes. In the present study, we further characterized effects of activin A on B cell growth and IgA expression. We found that activin A did not have effect on LPS-stimulated cell viability. In parallel, CFSE staining analysis revealed that activin A did not alter cell division. An increase of IgA secretion by activin A was completely abrogated by anti-activin A Ab but not by anti-TGFbeta1 Ab. In the same conditions, no other isotypes are significantly affected by each antibody treatment. Finally, activin A, as similar to TGF-beta1, increased IgA secretion by mesenteric lymph node cells. These results suggest that activin A can specifically stimulate IgA response, independent of TGF-beta in the gut.

Keyword

activin A; TGF-beta; IgA; mesenteric lymph node; CFSE

MeSH Terms

Activins
Animals
B-Lymphocytes
Cell Division
Cell Survival
Fluoresceins
Immunoglobulin A
Lymph Nodes
Mice
Succinimides
Transforming Growth Factor beta
Transforming Growth Factor beta1
Activins
Fluoresceins
Immunoglobulin A
Succinimides
Transforming Growth Factor beta
Transforming Growth Factor beta1