J Vet Sci.  2010 Jun;11(2):161-163. 10.4142/jvs.2010.11.2.161.

Molecular identification of the vaccine strain from the inactivated oil emulsion H9N2 low pathogenic avian influenza vaccine

• Choi JG
• Lee YJ
• Kim JY
• Kim YH
• Paek MR
• Yang DK
• Son SW
• Kim JH

• KMID: 1110864
• DOI: http://doi.org/10.4142/jvs.2010.11.2.161

Abstract

In order to control the H9N2 subtype low pathogenic avian influenza (LPAI), an inactivated vaccine has been used in Korea since 2007. The Korean veterinary authority permitted the use of a single H9N2 LPAI vaccine strain to simplify the evolution of the circulating virus due to the immune pressure caused by the vaccine use. It is therefore important to determine the suitability of the vaccine strain in the final inactivated oil emulsion LPAI vaccine. In this study, we applied molecular rather than biological methods to verify the suitability of the vaccine strain used in commercial vaccines and successfully identified the strain by comparing the nucleotide sequences of the hemagglutinin and neuraminidase genes with that of the permitted Korean LPAI vaccine strain. It is thought that the method used in this study might be successfully applied to other viral genes of the LPAI vaccine strain and perhaps to other veterinary oil emulsion vaccines.

Keyword

H9N2 avian influenza; identification of vaccine strain; oil emulsion vaccine

MeSH Terms

Animals
Base Sequence
Birds
DNA, Viral/chemistry/genetics
Hemagglutinin Glycoproteins, Influenza Virus/chemistry/genetics
Influenza A Virus, H9N2 Subtype/genetics/*immunology
Influenza Vaccines/genetics/*immunology
Influenza in Birds/*immunology/prevention & control/virology
Molecular Sequence Data
Neuraminidase/chemistry/genetics
Polymerase Chain Reaction/veterinary
Republic of Korea
Sequence Alignment
Vaccines, Inactivated/genetics/immunology

Figure

• 

  Fig. 1 Comparison of the nucleotide sequences of the hemagglutinin (A) and neuraminidase (B) genes of the vaccine parental strain (01310/CE3), vaccine strain (01310/CE20), five commercial LPAI vaccines (vaccine A to E), and representative Korean H9N2 LPAI viruses (field isolates). The GenBank accession number for the hemagglutinin and neuraminidase genes of A/chicken/Korea/MS96/1996 (MS96/96) are AF203008 and AF203786, respectively. Q99/06, GJ84/07 and GJ180/08 were sequenced in this study. Dots indicate residues identical to 01310/CE3 and dashes stand for missing nucleotides. The primer sequences are indicated with italic bold fonts. Nucleotide sequences for the additional potential N-linked glycosylation site are indicated with underlines.

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