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Evaluation of porcine intestinal organoids as an in vitro model for mammalian orthoreovirus 3 infection

Lee SA, Lee HJ, Gu NY, Park YR, Kim EJ, Kang SJ, Hyun BH, Yang DK

Background: Mammalian orthoreovirus type 3 (MRV3), which is responsible for gastroenteritis in many mammalian species including pigs, has been isolated from piglets with severe diarrhea. However, the use of pig-derived...
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Evaluation of commercial immunochromatography test kits for diagnosing canine parvovirus

Hyeon LS, Yang DK, Kim EJ, Park YR, Lee HJ, Hyun BH

Rapid immunochromatography test (RICT) kits are commonly used for the diagnosis of canine parvovirus (CPV) because of their rapid turnaround time, simplicity, and ease of use. However, the potential for...
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Rabies neutralizing antibody titers in Korean dogs and cats intended for overseas travel

Yang DK, Park YR, Kim HH, Kim EJ, Lee HJ, Cheong YJ, Hyun BH

Animals imported from abroad are a cause of rabies outbreaks in many countries. Therefore, rabies serology testing for dogs and cats traveling abroad is an important measure to reduce the...
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Immunogenicity of a new inactivated vaccine against feline panleukopenia virus, calicivirus, and herpesvirus-1 for cats

Yang DK, Park YR, Kim EJ, Lee HJ, Oh S, Hyun BH

Feline panleukopenia virus (FPV), feline calicivirus (FCV), and feline herpesvirus type-1 (FHV-1) are major infectious pathogens in cats. We evaluated the immunogenicity of a new vaccine containing inactivated FPV, two...
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Serological survey for Getah virus in domestic pigs of South Korea

Park YR, Kim EJ, Lee HJ, Hyun BH, Yang DK

Several outbreaks of Getah virus (GETV) have been reported in horses and pigs, causing large economic losses. However, there have been no reports describing serological survey for GETV in South...
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Incidence and sero-surveillance of feline viruses in Korean cats residing in Gyeonggi-do

Yang DK, Park YR, Kim Ej, Lee HJ, Shin KS, Kim JH, Lee K, Hyun BH

Incidences of major feline viral diseases provide basic information for preventing viral disease in cats. Despite the growing interest in feline viral diseases, sero-surveillances have been lacking. In this study,...
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Antiviral effect of 18-mer-peptide (1b-4/21-C12) on Japanese encephalitis virus and Akabane virus

Yang DK, Park YR, Kwon YD, Kim HH, Hyun BH

Japanese encephalitis virus (JEV) and Akabane virus (AKAV) are mosquito-borne viruses that cause encephalitis and reproductive disorders in horses and cattle, respectively. There is no treatment for JEV or AKAV...
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Isolation and molecular characterization of feline panleukopenia viruses from Korean cats

Yang DK, Park YR, Park Y, An S, Choi SS, Park J, Hyun BH

Feline panleukopenia virus (FPV) causes fatal leukopenia and severe hemorrhagic diarrhea in cats. Although FPV isolates have been reported worldwide from several animals, the biological and genetic features of South...
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Generation of a recombinant rabies virus expressing green fluorescent protein for a virus neutralization antibody assay

Yang DK, Kim HH, Park YR, Yoo JY, Park Y, Park J, Hyun BH

Background: Fluorescent antibody virus neutralization (FAVN) test is a standard assay for quantifying rabies virus-neutralizing antibody (VNA) in serum. However, a safer rabies virus (RABV) should be used in the...
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Expression of the VP2 protein of feline panleukopenia virus in insect cells and use thereof in a hemagglutination inhibition assay

Yang DK, Park Y, Park YR, Yoo JY, An S, Park J, Hyun BH

Feline panleukopenia virus (FPV) causes leukopenia and severe hemorrhagic diarrhea, killing 50% of naturally infected cats. Although intact FPV can serve as an antigen in the hemagglutination inhibition (HI) test,...
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Isolation and identification of mammalian orthoreovirus type 3 from a Korean roe deer (Capreolus pygargus)

Yang DK, An S, Park Y, Yoo JY, Park YR, Park J, Kim JT, Ahn S, Hyun BH

Mammalian reovirus (MRV) causes respiratory and intestinal disease in mammals. Although MRV isolates have been reported to circulate in several animals, there are no reports on Korean MRV isolates from...
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Immunogenicity of an inactivated rabies vaccine for animals derived from the recombinant ERAGS strain

Yang DK, Kim HH, Park YR, Yoo JY, Park Y, An S, Hyun BH

Purpose The aims of the present study were to evaluate the immunogenicity of an inactivated rabies vaccine based on the ERAGS strain Materials and Methods The ERAGS virus propagated in Vero cells was...
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Isolation and Molecular Characterization of Feline Herpesvirus 1 from Naturally Infected Korean Cats

Yang DK, Kim HH, Park YR, Yoo JY, Choi SS, Park Y, An S, Park J, Kim J, Kim HJ, Lee J, Hyun BH

Feline herpesvirus type 1 (FHV-1) causes respiratory and ocular disease in cats. Although isolates of FHV-1 circulating in cats have been reported worldwide, Korean FHV-1 isolates and their features have not...
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Biological and molecular characterization of feline caliciviruses isolated from cats in South Korea

Yang DK, Park YR, Yoo JY, Choi SS, Park Y, An S, Park J, Kim HJ, Kim J, Kim HH, Hyun BH

Feline calicivirus (FCV) infection results in a common upper respiratory disease associated with oral ulceration in cats. Although FCV infection has been reported in cats worldwide, the biologic and genetic features...
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Isolation and molecular characterizations of canine distemper virus from a naturally infected Korean dog using Vero cells expressing dog signaling lymphocyte activation molecule

Yang DK, Kim HH, Lee S, Yoon YS, Park J, Oh D, Yoo JY, Ji M, Han B, Oh S, Hyun BH

Background: Canine distemper virus (CDV) infection results in high morbidity and mortality in dogs. There has been no report about Isolation of Korean CDV since 1980 in Korea. Objectives: To investigate...
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Genotype-specific neutralizing antibody titers against Japanese encephalitis virus genotypes 1 and 3 in horses immunized with a genotype 3 vaccine

Kim HH, Yang DK, Ji M, Lee SK, Hyun BH

Purpose: Japanese encephalitis is one of the most important mosquito-borne and zoonotic diseases in Asia and the Pacific region. Although the dominant Japanese encephalitis virus (JEV) genotype has shifted from...
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Development of indirect ELISA for the detection of canine adenovirus type 2 antibodies in dog sera

Yang DK, Kim HH, Lee S, Oh D, Yoo JY, Hyun BH

Background: Canine adenovirus type 2 (CAV-2) induces infectious laryngotracheitis in members of the family Canidae, including dogs. To date, no ELISA kits specific for CAV-2 antibody have been commercialized for...
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Evaluation of Commercial Immunochromatographic Test Kits for the Detection of Canine Distemper Virus

Yang DK, Kim HH, Lee J, Ji M, Oh S, Lee HS, Hyun BH

The rapid diagnosis of canine distemper virus (CDV) helps to determine the treatment of dogs in veterinary clinics. We evaluated the performance of seven commercial rapid immunochromatographic test (RICT) kits...
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Establishment of multiplex RT-PCR for differentiation between rabies virus with and that without mutation at position 333 of glycoprotein

Yang DK, Kim HH, Lee S, Yoo JY

Rabid raccoon dogs (Nyctereutes procyonoides koreensis) have been responsible for animal rabies in South Korea since the 1990s. A recombinant rabies vaccine strain, designated as ERAGS, was constructed for use...
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Development of an Indirect ELISA Featuring Plates Coated with Column Chromatographically Purified Canine Adenovirus Type-1 Antigen

Yang DK, Kim HH, Lee S, Ji M, Han BH, Oh S, Hyun BH

Canine adenovirus type 1 (CAV-1) causes infectious hepatitis in members of the family Canidae, including dogs. An indirect enzyme-linked immunosorbent assay (I-ELISA) that detects CAV-1 antibodies is required for large-throughput...
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