J Vet Sci.  2010 Mar;11(1):51-58. 10.4142/jvs.2010.11.1.51.

Antimutagenic and anticarcinogenic effect of methanol extracts of Petasites japonicus Maxim leaves

• Kang HG
• Jeong SH
• Cho JH

• KMID: 1106157
• DOI: http://doi.org/10.4142/jvs.2010.11.1.51

Abstract

The methanol extract from the leaves of Petasites japonicus Maxim (PJ) was studied for its (anti-)mutagenic effect with the SOS chromotest and reverse mutation assay. The (anti-)carcinogenic effects were evaluated by the cytotoxicity on human cancer line cells and by the function and the expression of gap junctions in rat liver epithelial cell. PJ extracts significantly decreased spontaneous beta-galactosidase activity and beta-galactosidase activity induced by a mutagen, ICR, in Salmonella (S.) typhimurium TA 1535/pSK 1002. All doses of the extract (0.08-100 mg/plate) decreased the reversion frequency induced by benzo (alpha)pyrene (BaP) in S. typhimurium TA 98. It decreased not only the spontaneous reversion frequency but also that induced by BaP in S. typhimurium TA 100. PJ extract showed greater cytotoxic effects on human stomach, colon and uterus cancer cells than on other cancer cell types and normal rat liver epithelial cells. Dye transfers though gap junctions were significantly increased by PJ extracts at concentrations greater than 200 microg/mL and the inhibition of dye transfer by 12-O-tetradecanoylphorobol-13-acetate (TPA) was obstructed in all concentrations of PJ. PJ significantly increased the numbers of gap junction protein connexin 43, and increased the protein expression decreased by TPA in a dose-dependent manner. Based on these findings, PJ is suggested to contain antimutagenic and anticarcionogenic compounds.

Keyword

anticarcinogenicity; antimutagenicity; gap junction; Petasites japonicus Maxim

MeSH Terms

Animals
Cell Line, Tumor
Cell Survival/*drug effects
Formazans/chemistry
Gap Junctions/*metabolism
Humans
Mutagenicity Tests
Petasites/*metabolism
Plant Extracts/*pharmacology
Plant Leaves/metabolism
Rats
Tetrazolium Salts/chemistry

Figure

• 

  Fig. 1 Gap junctional intercellular communication assayed with Lucifer yellow CH dye solution in rat liver epithelial cells. The cells were exposed to 0.1% methanol (A), 0.01 µg/mL 12-O-tetradecanoylphorobol-13-acetate (TPA) (B) and 0.01 µg/mL TPA + 40 µg/mL Petasites japonicus Maxim (C) extract for 1 h. The cells were observed under epifluorescence microscope. ×100.

• 

  Fig. 2 Gap junction proteins in rat liver epithelial cells. The cells were exposed to 0.1% methanol (A), 0.01 µg/mL TPA (B) and 0.01 µg/mL TPA + 40 µg/mL Petasites japonicus Maxim (C) extract for 1 h. The cells were observed under epifluorescence microscope. ×1,000.

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