Nat Prod Sci.  2017 Dec;23(4):270-273. 10.20307/nps.2017.23.4.270.

Quantitative Determination of Bakkenolide D in Petasites japonicus and Farfugium japonicum by HPLC/UV

• Quilantang NG
• Lee KH
• Lee DG
• Lee JS
• Cho E
• Kim HY
• Lee S

• KMID: 2401579
• DOI: http://doi.org/10.20307/nps.2017.23.4.270

Abstract

A quantitative analysis of bakkenolide D in the different parts of Petasites japonicus and Farfugium japonicum was performed by HPLC. A gradient HPLC elution system with a mobile phase consisting of water:acetonitrile solution (20:80 to 0:100 for 45 min) was followed and an INNO C₁₈ column was used for the chromatographic separation. The injection volume, flow rate, and UV detection were 10 µL, 1 mL/min, and 290 nm, respectively. Results show that both species showed the highest amount of bakkenolide D in the roots being 107.203 and 166.103 mg/g for P. japonicas and F. japonicum, respectively. Content analysis on the different parts of both plants displayed remarkably lower values which ranged from 0.403 - 4.419 and 7.252 - 32.614 mg/g for P. japonicas and F. japonicum, respectively. The results show that the roots of both plants are rich in bakkenolide D showing a promising use in the development of nutraceuticals and industrial application of the compound.

Keyword

Bakkenolide D; Petasites japonicus; Farfugium japonicum; HPLC/UV

MeSH Terms

Chromatography, High Pressure Liquid
Dietary Supplements
Petasites*

Figure

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  Fig. 1. Structure of bakkenolide D.

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  Fig. 2. HPLC chromatograms of bakkenolide D (A) and the MeOH extracts of P. japonicus (B) and F. japonicum (C).

Reference

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