Nat Prod Sci.  2017 Dec;23(4):270-273. 10.20307/nps.2017.23.4.270.

Quantitative Determination of Bakkenolide D in Petasites japonicus and Farfugium japonicum by HPLC/UV

  • 1Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, Korea.
  • 2Department of Food Science and Nutrition & Kimchi Research Institute, Pusan National University, Busan 46241, Korea.
  • 3Department of Food Science, Gyeongnam National University of Science and Technology, Jinju 52725, Korea.


A quantitative analysis of bakkenolide D in the different parts of Petasites japonicus and Farfugium japonicum was performed by HPLC. A gradient HPLC elution system with a mobile phase consisting of water:acetonitrile solution (20:80 to 0:100 for 45 min) was followed and an INNO C₁₈ column was used for the chromatographic separation. The injection volume, flow rate, and UV detection were 10 µL, 1 mL/min, and 290 nm, respectively. Results show that both species showed the highest amount of bakkenolide D in the roots being 107.203 and 166.103 mg/g for P. japonicas and F. japonicum, respectively. Content analysis on the different parts of both plants displayed remarkably lower values which ranged from 0.403 - 4.419 and 7.252 - 32.614 mg/g for P. japonicas and F. japonicum, respectively. The results show that the roots of both plants are rich in bakkenolide D showing a promising use in the development of nutraceuticals and industrial application of the compound.


Bakkenolide D; Petasites japonicus; Farfugium japonicum; HPLC/UV

MeSH Terms

Chromatography, High Pressure Liquid
Dietary Supplements


  • Fig. 1. Structure of bakkenolide D.

  • Fig. 2. HPLC chromatograms of bakkenolide D (A) and the MeOH extracts of P. japonicus (B) and F. japonicum (C).



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