Korean J Parasitol.
2006 Mar;44(1):49-54. 10.3347/kjp.2006.44.1.49.
Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis of Plasmodium falciparum infection
- Affiliations
-
- 1Division of Tropical and Endemic Parasitic Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul 122-701, Korea. nihkim@nih.go.kr
- 2Parasitology Division, Department of Medical Research (Upper Myanmar), Mandalay, Myanmar.
- 3Department of Molecular Parasitology, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea.
- 4Department of Clinical Pathology, Ansan College, Ansan 425-701, Korea.
- KMID: 1091836
- DOI: http://doi.org/10.3347/kjp.2006.44.1.49
Abstract
- In order to develop tools for an early serodiagnosis of Plasmodium falciparum infection, we evaluated the usefulness of P. falciparum liver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion of LSA-3 gene was cloned, and its recombinant protein (rLSA-3) was expressed in Escherichia coli and purified by column chromatography. The purified rLSA-3 and 120 test blood/serum samples collected from inhabitants in malaria-endemic areas of Mandalay, Myanmar were used for this study. In microscopic examinations of blood samples, P. falciparum positive rate was 39.1% (47/120) in thin smear trials, and 33.3% (40/120) in thick smear trials. Although the positive rate associated with the rLSA-3 (30.8%) was lower than that of the blood stage antigens (70.8%), rLSA-3 based enzyme-linked immunosorbent assay could detect 12 seropositive cases (10.0%), in which blood stage antigens were not detected. These results indicate that the LSA-3 is a useful antigen for an early serodiagnosis of P. falciparum infection.
Keyword
MeSH Terms
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Recombinant Proteins/biosynthesis/genetics/*immunology
Plasmodium vivax/isolation & purification
Plasmodium falciparum/*immunology
Molecular Sequence Data
Malaria, Falciparum/blood/*diagnosis
Humans
Genes, Protozoan/genetics/immunology
Fluorescent Antibody Technique, Direct/methods
Escherichia coli/genetics
Enzyme-Linked Immunosorbent Assay/methods
Early Diagnosis
DNA, Protozoan/chemistry
DNA Primers/chemistry
Cloning, Molecular/methods
Base Sequence
Antigens, Protozoan/biosynthesis/chemistry/genetics/*immunology
Animals
Amino Acid Sequence
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