Exp Mol Med.  2005 Oct;37(5):379-390.

Characterization of newly established oral cancer cell lines derived from six squamous cell carcinoma and two mucoepidermoid carcinoma cells

Affiliations
  • 1Department of Oral Pathology, Oral Cancer Research Institute, Brain Korea 21 Project for Medical Science Yonsei University, School of Dentistry, Seoul 120-752, Korea. jiyook@yumc.yonsei.ac.kr
  • 2Department of Dental Hygiene NamSeoul University Cheon-Ahn 330-707, Korea.

Abstract

Since genetic abnormalities of human cancer are greatly geographically dependent, cultural and environmental backgrounds are thought to be closely related to the carcinogenic process. In the present study, eight human cell lines were established by culture from untreated carcinomas of the oral cancer, of which five were from primary oral squamous cell carcinomas (OSC), one from a mucoepidermoid carcinoma (MEC) and one each originating from metastatic OSC and MEC. All the studied tumor lines grew as monolayers, and showed: i) an epithelial origin by the presence of cytokeratin, and ii) tumorigenic potential in nude mice. Western blot analysis revealed i) over expression of EGFR in six of the cell lines ii) decreased expression of E- cadherin in six cell lines compared to normal human oral mucosa. A mutational analysis showed: point mutations of p53 at exon 7, with transversion, and at exon 8, with transition. These well-characterized human YD cell lines should serve as useful tools in the study of the molecular pathogenesis and biological characteristics of head and neck cancer cells, and in the future testing of new therapeutic reagents for oral cancer.

Keyword

carcinoma, mucoepideermoid; cadherins; carcinoma, squamous cell; cell line, tumor; mouth neoplasms; p53; receptor, epidermal growth factor
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